Microbiota metabolite short-chain fatty acids facilitate mucosal adjuvant activity of cholera toxin through GPR43

Wenjing Yang, Yi Xiao, Xiangsheng Huang, Feidi Chen, Mingming Sun, Anthony J. Bilotta, Leiqi Xu, Yao Lu, Suxia Yao, Qihong Zhao, Zhanju Liu, Yingzi Cong

Research output: Contribution to journalArticle

Abstract

The gut microbiota has been shown critical for mucosal adjuvant activity of cholera toxin (CT), a potent mucosal adjuvant. However, the mechanisms involved remain largely unknown. In this study, we report that depletion of gut bacteria significantly decreased mucosal and systemic Ab responses in mice orally immunized with OVA and CT. Feeding mice short-chain fatty acids (SCFAs) promoted Ab responses elicited by CT, and, more importantly, rescued Ab responses in antibiotic-treated mice. In addition, mice deficient in GPR43, a receptor for SCFAs, showed impaired adjuvant activity of CT. Administering CT did not promote SCFA production in the intestines; thus, SCFAs facilitated but did not directly mediate the adjuvant activity of CT. SCFAs promoted B cell Ab production by promoting dendritic cell production of BAFF and ALDH1a2, which induced B cell expression of IFN regulatory factor 4, Blimp1, and XBP1, the plasma B cell differentiation-related genes. Furthermore, when infected with Citrobacter rodentium, GPR43-/- mice exhibited decreased Ab responses and were more susceptible to infection, whereas the administration of SCFAs promoted intestinal Ab responses in wild-type mice. Our study thereby demonstrated a critical role of gut microbiota and their metabolite SCFAs in promoting mucosal adjuvant activity of CT through GPR43.

Original languageEnglish (US)
Pages (from-to)282-292
Number of pages11
JournalJournal of Immunology
Volume203
Issue number1
DOIs
StatePublished - Jan 1 2019

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Volatile Fatty Acids
Cholera Toxin
Microbiota
B-Lymphocytes
Citrobacter rodentium
Plasma Cells
Dendritic Cells
Intestines
Cell Differentiation
Anti-Bacterial Agents
Bacteria
Infection
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Microbiota metabolite short-chain fatty acids facilitate mucosal adjuvant activity of cholera toxin through GPR43. / Yang, Wenjing; Xiao, Yi; Huang, Xiangsheng; Chen, Feidi; Sun, Mingming; Bilotta, Anthony J.; Xu, Leiqi; Lu, Yao; Yao, Suxia; Zhao, Qihong; Liu, Zhanju; Cong, Yingzi.

In: Journal of Immunology, Vol. 203, No. 1, 01.01.2019, p. 282-292.

Research output: Contribution to journalArticle

Yang, W, Xiao, Y, Huang, X, Chen, F, Sun, M, Bilotta, AJ, Xu, L, Lu, Y, Yao, S, Zhao, Q, Liu, Z & Cong, Y 2019, 'Microbiota metabolite short-chain fatty acids facilitate mucosal adjuvant activity of cholera toxin through GPR43', Journal of Immunology, vol. 203, no. 1, pp. 282-292. https://doi.org/10.4049/jimmunol.1801068
Yang, Wenjing ; Xiao, Yi ; Huang, Xiangsheng ; Chen, Feidi ; Sun, Mingming ; Bilotta, Anthony J. ; Xu, Leiqi ; Lu, Yao ; Yao, Suxia ; Zhao, Qihong ; Liu, Zhanju ; Cong, Yingzi. / Microbiota metabolite short-chain fatty acids facilitate mucosal adjuvant activity of cholera toxin through GPR43. In: Journal of Immunology. 2019 ; Vol. 203, No. 1. pp. 282-292.
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