Microbiota of the Oropharynx and Endoscope Compared to the Esophagus

Ikenna Okereke, Aaron L. Miller, Catherine F. Hamilton, Adam L. Booth, Gabriel Reep, Clark L. Andersen, Sandy T. Reynolds, Richard Pyles

Research output: Contribution to journalArticle

Abstract

The role of the microflora in the development of esophageal disease is still largely unknown and is being investigated in more detail. Our goal was to determine how the microbiota levels of endoscope and uvular swabs compared to the levels of tissue biopsies along various points of the esophagus. 17 patients with Barrett’s esophagus agreed to participate in the study. Biopsies of esophageal mucosa were taken from the (1) proximal esophagus, (2) mid-esophagus, (3) distal esophagus, and (4) Barrett’s esophagus. Swabs were also taken from the uvula and the endoscope. Throughout the esophagus, 17 bacterial genera were detected from the samples. The microflora pattern obtained from the uvula and endoscopic swabs did not correlate well with mucosal biopsies along any aspect of the esophagus. There were statistically significant differences in the levels and proportions of bacteria found when comparing the uvula swab to the esophageal biopsies and when comparing the endoscope swab to the esophageal biopsies. Obtaining a simple swab of the uvula or endoscope itself appears to be a poor substitute for tissue biopsy of esophageal mucosa when evaluating microflora patterns. When performing microflora studies of the esophagus, mucosal biopsies should be used for analysis.

Original languageEnglish (US)
Article number10201
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Oropharynx
Endoscopes
Microbiota
Esophagus
Uvula
Biopsy
Barrett Esophagus
Esophageal Diseases
Bacteria

ASJC Scopus subject areas

  • General

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Microbiota of the Oropharynx and Endoscope Compared to the Esophagus. / Okereke, Ikenna; Miller, Aaron L.; Hamilton, Catherine F.; Booth, Adam L.; Reep, Gabriel; Andersen, Clark L.; Reynolds, Sandy T.; Pyles, Richard.

In: Scientific reports, Vol. 9, No. 1, 10201, 01.12.2019.

Research output: Contribution to journalArticle

Okereke, Ikenna ; Miller, Aaron L. ; Hamilton, Catherine F. ; Booth, Adam L. ; Reep, Gabriel ; Andersen, Clark L. ; Reynolds, Sandy T. ; Pyles, Richard. / Microbiota of the Oropharynx and Endoscope Compared to the Esophagus. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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