Microglial cells from psychologically stressed mice as an accelerator of cerebral cryptococcosis

Masae Shimoda, Vickie C. Jones, Makiko Kobayashi, Fujio Suzuki

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Severe stress decreases the resistance of hosts exposed to microbial infections. As compared with two groups of control mice (normal mice, food-and-water-deprived mice [FWD mice]), restraint-stressed mice (RST mice) were shown to be greatly susceptible to intracerebral growth of Cryptococcus neoformans. The susceptibility of FWD mice to cerebral cryptococcosis increased to the level shown in RST mice, when these groups of mice were inoculated with microglial cells from the brains of RST mice. However, the susceptibility of FWD mice to cerebral cryptococcosis was not influenced by the adoptive transfer of microglial cells from normal mice or FWD mice. Microglial cells from RST mice produced CC-chemokine ligand-2 (CCL-2/monocyte chemoattractant protein 1), but not microglial cells from FWD mice. The resistance of RST mice to cerebral cryptococcosis was improved to the extent shown in FWD mice, when they were treated with anti-CCL-2 antibody. However, the susceptibility of normal mice and FWD mice to cerebral cryptococcosis increased to that shown in RST mice, when they were treated with rCCL-2. Microglial cells from RST mice were discriminated from the same cell preparations derived from FWD mice by their abilities to produce CCL-2, to phagocytize C. neoformans cells and to express Toll-like receptor 2. These results indicate that the resistance of RST mice to cerebral cryptococcosis is diminished by CCL-2 produced by microglial cells that are influenced by restraint stress.

Original languageEnglish (US)
Pages (from-to)551-556
Number of pages6
JournalImmunology and Cell Biology
Volume84
Issue number6
DOIs
StatePublished - Dec 2006

    Fingerprint

Keywords

  • CC-chemokine ligand-2
  • Cryptococcus neoformans
  • Microglial cell
  • Stress

ASJC Scopus subject areas

  • Immunology
  • Clinical Biochemistry
  • Cell Biology

Cite this