MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome

QiQi Zhou, Wiley W. Souba, Carlo M. Croce, G. Nicholas Verne

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Background: The molecular mechanisms underlying the pathophysiology of irritable bowel syndrome (IBS) are poorly understood. One mechanism may involve increased intestinal permeability that is reversed with glutamine supplementation. Our goal was to evaluate the expression of glutamine synthetase and its complementary miRNA in blood microvesicles and gut tissues of IBS patients with increased intestinal membrane permeability. Methods: We evaluated 19 diarrhoea-predominant IBS patients and 10 controls for intestinal membrane permeability using the lactulose/mannitol method. miRNA expression was evaluated in blood microvesicles and gut tissue. To further confirm the relationship between miRNA and glutamine synthetase expression, cell culture experiments were conducted. Glutamine synthetase was also evaluated in the gut tissues of patients. Results: A subset of patients with IBS (8/19, 42%) had increased intestinal membrane permeability and decreased glutamine synthetase expression compared to patients with IBS normal membrane permeability, and to controls. Expression of miR-29a was increased in blood microvesicles, small bowel and colon tissues of IBS patients with increased intestinal membrane permeability. Increased intestinal permeability was modulated by miR-29a which has a complementary site in the 3′-UTR of the GLUL gene. Conclusions: The results support the conclusion that GLUL regulates intestinal membrane permeability and miR-29a regulates both GLUL and intestinal membrane permeability. The data suggests that miR-29a effects on intestinal membrane permeability may be due to its regulation of GLUL. Targeting this signalling pathway could lead to a new therapeutic approach to the treatment of patients with IBS, especially because small molecules that mimic or inhibit miRNA-based mechanisms are readily available.

Original languageEnglish (US)
Pages (from-to)775-784
Number of pages10
JournalGut
Volume59
Issue number6
DOIs
StatePublished - Jun 2010

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Irritable Bowel Syndrome
MicroRNAs
Permeability
Membranes
Glutamate-Ammonia Ligase
Lactulose
3' Untranslated Regions
Mannitol
Glutamine
Diarrhea
Colon
Cell Culture Techniques

ASJC Scopus subject areas

  • Gastroenterology

Cite this

MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome. / Zhou, QiQi; Souba, Wiley W.; Croce, Carlo M.; Verne, G. Nicholas.

In: Gut, Vol. 59, No. 6, 06.2010, p. 775-784.

Research output: Contribution to journalArticle

Zhou, QiQi ; Souba, Wiley W. ; Croce, Carlo M. ; Verne, G. Nicholas. / MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome. In: Gut. 2010 ; Vol. 59, No. 6. pp. 775-784.
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abstract = "Background: The molecular mechanisms underlying the pathophysiology of irritable bowel syndrome (IBS) are poorly understood. One mechanism may involve increased intestinal permeability that is reversed with glutamine supplementation. Our goal was to evaluate the expression of glutamine synthetase and its complementary miRNA in blood microvesicles and gut tissues of IBS patients with increased intestinal membrane permeability. Methods: We evaluated 19 diarrhoea-predominant IBS patients and 10 controls for intestinal membrane permeability using the lactulose/mannitol method. miRNA expression was evaluated in blood microvesicles and gut tissue. To further confirm the relationship between miRNA and glutamine synthetase expression, cell culture experiments were conducted. Glutamine synthetase was also evaluated in the gut tissues of patients. Results: A subset of patients with IBS (8/19, 42{\%}) had increased intestinal membrane permeability and decreased glutamine synthetase expression compared to patients with IBS normal membrane permeability, and to controls. Expression of miR-29a was increased in blood microvesicles, small bowel and colon tissues of IBS patients with increased intestinal membrane permeability. Increased intestinal permeability was modulated by miR-29a which has a complementary site in the 3′-UTR of the GLUL gene. Conclusions: The results support the conclusion that GLUL regulates intestinal membrane permeability and miR-29a regulates both GLUL and intestinal membrane permeability. The data suggests that miR-29a effects on intestinal membrane permeability may be due to its regulation of GLUL. Targeting this signalling pathway could lead to a new therapeutic approach to the treatment of patients with IBS, especially because small molecules that mimic or inhibit miRNA-based mechanisms are readily available.",
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