TY - JOUR
T1 - Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins
T2 - Usefulness as screening test for large numbers of specimens including hybridoma supernates
AU - McKendall, Robert R.
AU - Woo, Wayne
N1 - Funding Information:
This work was supported by research funds from the Veterans Administration, The Kroc Foundation and the University of California Academic Senate. We thank Ida Ransby for excellent preparation of the manuscript.
PY - 1984/9/4
Y1 - 1984/9/4
N2 - Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.
AB - Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.
KW - HSV-1 polypeptides
KW - hybridoma screening
KW - microtiter radioimmunoprecipitation
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U2 - 10.1016/0022-1759(84)90014-0
DO - 10.1016/0022-1759(84)90014-0
M3 - Article
C2 - 6088637
AN - SCOPUS:0021207291
SN - 0022-1759
VL - 72
SP - 461
EP - 469
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -