Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins: Usefulness as screening test for large numbers of specimens including hybridoma supernates

Robert R. McKendall; Wayne Woo

doi:10.1016/0022-1759(84)90014-0

Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins: Usefulness as screening test for large numbers of specimens including hybridoma supernates

Robert R. McKendall
, Wayne Woo

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.

Original language	English (US)
Pages (from-to)	461-469
Number of pages	9
Journal	Journal of Immunological Methods
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(84)90014-0
State	Published - Sep 4 1984
Externally published	Yes

Keywords

HSV-1 polypeptides
hybridoma screening
microtiter radioimmunoprecipitation

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(84)90014-0

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Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins: Usefulness as screening test for large numbers of specimens including hybridoma supernates. / McKendall, Robert R.; Woo, Wayne.
In: Journal of Immunological Methods, Vol. 72, No. 2, 04.09.1984, p. 461-469.

Research output: Contribution to journal › Article › peer-review

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title = "Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins: Usefulness as screening test for large numbers of specimens including hybridoma supernates",

abstract = "Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.",

keywords = "HSV-1 polypeptides, hybridoma screening, microtiter radioimmunoprecipitation",

author = "McKendall, \{Robert R.\} and Wayne Woo",

note = "Funding Information: This work was supported by research funds from the Veterans Administration, The Kroc Foundation and the University of California Academic Senate. We thank Ida Ransby for excellent preparation of the manuscript.",

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doi = "10.1016/0022-1759(84)90014-0",

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T2 - Usefulness as screening test for large numbers of specimens including hybridoma supernates

AU - McKendall, Robert R.

AU - Woo, Wayne

N1 - Funding Information: This work was supported by research funds from the Veterans Administration, The Kroc Foundation and the University of California Academic Senate. We thank Ida Ransby for excellent preparation of the manuscript.

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N2 - Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.

AB - Immunoprecipitation of radiolabeled polypeptides from complex mixtures of proteins was performed in polystyrene microtiter plates using staphylococcus protein A and various antibody preparations. The method is (1) rapid, (2) uses multichannel micropipettor technology, (3) handles large numbers of specimen easily, (4) requires very small volumes of antigen and antibody (5-50 μl), (5) provides replicates for statistical analysis and (6) allows recovery of precipitated proteins for direct SDS-PAGE analysis of precipitated proteins. We have shown it is useful as a test to screen large numbers of sera or to characterize monoclonal antibody-containing samples.

KW - HSV-1 polypeptides

KW - hybridoma screening

KW - microtiter radioimmunoprecipitation

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Microtiter radioimmunoprecipitation assay of HSV-1 polypeptides with recovery and SDS-PAGE analysis of precipatated proteins: Usefulness as screening test for large numbers of specimens including hybridoma supernates

Abstract

Keywords

ASJC Scopus subject areas

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