Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines

C. Brent Chesson, Rojelio Elias Alvarado, Jai S. Rudra

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Self-assembling peptides spontaneously associate into functional supramolecular scaffolds, which have found numerous biomedical applications. These molecular assemblies have applications in nerve regeneration, wound healing, and both prophylactic and therapeutic vaccination. They can also be useful tools for proliferation assays, sustained culture of difficult cell lines, or activation of cell lines for immunoassays. This protocol will describe the basic peptide synthesis and purification of model self-assembling peptide immunogen and methods for vaccinating mice, collecting lymph nodes, and stimulating cells ex vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages249-259
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1777
ISSN (Print)1064-3745

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Subunit Vaccines
Microwaves
Peptides
Cell Line
Nerve Regeneration
Immunoassay
Wound Healing
Vaccination
Lymph Nodes
Therapeutics

Keywords

  • Nanofiber
  • Self-assembling peptides
  • Vaccine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Brent Chesson, C., Alvarado, R. E., & Rudra, J. S. (2018). Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines. In Methods in Molecular Biology (pp. 249-259). (Methods in Molecular Biology; Vol. 1777). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7811-3_15

Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines. / Brent Chesson, C.; Alvarado, Rojelio Elias; Rudra, Jai S.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 249-259 (Methods in Molecular Biology; Vol. 1777).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brent Chesson, C, Alvarado, RE & Rudra, JS 2018, Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1777, Humana Press Inc., pp. 249-259. https://doi.org/10.1007/978-1-4939-7811-3_15
Brent Chesson C, Alvarado RE, Rudra JS. Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 249-259. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7811-3_15
Brent Chesson, C. ; Alvarado, Rojelio Elias ; Rudra, Jai S. / Microwave-assisted synthesis and immunological evaluation of self-assembling peptide vaccines. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 249-259 (Methods in Molecular Biology).
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