Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene

Regie Lyn P Santos-Cortez, Diane S. Hutchinson, Nadim J. Ajami, Ma Rina T Reyes-Quintos, Ma Leah C Tantoco, Patrick John Labra, Sheryl Mae Lagrana, Melquiadesa Pedro, Erasmo Gonzalo d V Llanes, Teresa Luisa Gloria-Cruz, Abner L. Chan, Eva Maria Cutiongco-de la Paz, John W. Belmont, Tasnee Chonmaitree, Generoso T. Abes, Joseph F. Petrosino, Suzanne M. Leal, Charlotte M. Chiong

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Background: Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children. The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access. Methods: Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media, of whom 11 carry the A2ML1 duplication variant. Ear swabs were submitted for 16S rRNA gene sequencing. Results: Genotype-based differences in microbial richness, structure, and composition were identified, but were not statistically significant. Taxonomic analysis revealed that the relative abundance of the phyla Fusobacteria and Bacteroidetes, and genus Fusobacterium were nominally increased in carriers compared to non-carriers, but were non-significant after correction for multiple testing. We also detected rare bacteria including Oligella that was reported only once in the middle ear. Conclusions: These findings suggest that A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome. Knowledge of middle ear microbial profiles according to genetic background can be potentially useful for therapeutic and prophylactic interventions for otitis media and can guide public health interventions towards decreasing otitis media prevalence within the indigenous Filipino community.

    Original languageEnglish (US)
    Article number97
    JournalInfectious Diseases of Poverty
    Volume5
    Issue number1
    DOIs
    StatePublished - Nov 1 2016

    Fingerprint

    Microbiota
    Otitis Media
    Middle Ear
    Genes
    Ear
    Fusobacteria
    Fusobacterium
    Bacteroidetes
    Otitis
    rRNA Genes
    Public Health
    Genotype
    Bacteria
    Delivery of Health Care

    Keywords

    • A2ML1
    • Indigenous population
    • Microbiome
    • Middle ear
    • Oligella
    • Otitis media
    • Philippines

    ASJC Scopus subject areas

    • Public Health, Environmental and Occupational Health
    • Infectious Diseases

    Cite this

    Santos-Cortez, R. L. P., Hutchinson, D. S., Ajami, N. J., Reyes-Quintos, M. R. T., Tantoco, M. L. C., Labra, P. J., ... Chiong, C. M. (2016). Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene. Infectious Diseases of Poverty, 5(1), [97]. https://doi.org/10.1186/s40249-016-0189-7

    Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene. / Santos-Cortez, Regie Lyn P; Hutchinson, Diane S.; Ajami, Nadim J.; Reyes-Quintos, Ma Rina T; Tantoco, Ma Leah C; Labra, Patrick John; Lagrana, Sheryl Mae; Pedro, Melquiadesa; Llanes, Erasmo Gonzalo d V; Gloria-Cruz, Teresa Luisa; Chan, Abner L.; Cutiongco-de la Paz, Eva Maria; Belmont, John W.; Chonmaitree, Tasnee; Abes, Generoso T.; Petrosino, Joseph F.; Leal, Suzanne M.; Chiong, Charlotte M.

    In: Infectious Diseases of Poverty, Vol. 5, No. 1, 97, 01.11.2016.

    Research output: Contribution to journalArticle

    Santos-Cortez, RLP, Hutchinson, DS, Ajami, NJ, Reyes-Quintos, MRT, Tantoco, MLC, Labra, PJ, Lagrana, SM, Pedro, M, Llanes, EGDV, Gloria-Cruz, TL, Chan, AL, Cutiongco-de la Paz, EM, Belmont, JW, Chonmaitree, T, Abes, GT, Petrosino, JF, Leal, SM & Chiong, CM 2016, 'Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene', Infectious Diseases of Poverty, vol. 5, no. 1, 97. https://doi.org/10.1186/s40249-016-0189-7
    Santos-Cortez, Regie Lyn P ; Hutchinson, Diane S. ; Ajami, Nadim J. ; Reyes-Quintos, Ma Rina T ; Tantoco, Ma Leah C ; Labra, Patrick John ; Lagrana, Sheryl Mae ; Pedro, Melquiadesa ; Llanes, Erasmo Gonzalo d V ; Gloria-Cruz, Teresa Luisa ; Chan, Abner L. ; Cutiongco-de la Paz, Eva Maria ; Belmont, John W. ; Chonmaitree, Tasnee ; Abes, Generoso T. ; Petrosino, Joseph F. ; Leal, Suzanne M. ; Chiong, Charlotte M. / Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene. In: Infectious Diseases of Poverty. 2016 ; Vol. 5, No. 1.
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    abstract = "Background: Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children. The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access. Methods: Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media, of whom 11 carry the A2ML1 duplication variant. Ear swabs were submitted for 16S rRNA gene sequencing. Results: Genotype-based differences in microbial richness, structure, and composition were identified, but were not statistically significant. Taxonomic analysis revealed that the relative abundance of the phyla Fusobacteria and Bacteroidetes, and genus Fusobacterium were nominally increased in carriers compared to non-carriers, but were non-significant after correction for multiple testing. We also detected rare bacteria including Oligella that was reported only once in the middle ear. Conclusions: These findings suggest that A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome. Knowledge of middle ear microbial profiles according to genetic background can be potentially useful for therapeutic and prophylactic interventions for otitis media and can guide public health interventions towards decreasing otitis media prevalence within the indigenous Filipino community.",
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    T1 - Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene

    AU - Santos-Cortez, Regie Lyn P

    AU - Hutchinson, Diane S.

    AU - Ajami, Nadim J.

    AU - Reyes-Quintos, Ma Rina T

    AU - Tantoco, Ma Leah C

    AU - Labra, Patrick John

    AU - Lagrana, Sheryl Mae

    AU - Pedro, Melquiadesa

    AU - Llanes, Erasmo Gonzalo d V

    AU - Gloria-Cruz, Teresa Luisa

    AU - Chan, Abner L.

    AU - Cutiongco-de la Paz, Eva Maria

    AU - Belmont, John W.

    AU - Chonmaitree, Tasnee

    AU - Abes, Generoso T.

    AU - Petrosino, Joseph F.

    AU - Leal, Suzanne M.

    AU - Chiong, Charlotte M.

    PY - 2016/11/1

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    N2 - Background: Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children. The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access. Methods: Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media, of whom 11 carry the A2ML1 duplication variant. Ear swabs were submitted for 16S rRNA gene sequencing. Results: Genotype-based differences in microbial richness, structure, and composition were identified, but were not statistically significant. Taxonomic analysis revealed that the relative abundance of the phyla Fusobacteria and Bacteroidetes, and genus Fusobacterium were nominally increased in carriers compared to non-carriers, but were non-significant after correction for multiple testing. We also detected rare bacteria including Oligella that was reported only once in the middle ear. Conclusions: These findings suggest that A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome. Knowledge of middle ear microbial profiles according to genetic background can be potentially useful for therapeutic and prophylactic interventions for otitis media and can guide public health interventions towards decreasing otitis media prevalence within the indigenous Filipino community.

    AB - Background: Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children. The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access. Methods: Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media, of whom 11 carry the A2ML1 duplication variant. Ear swabs were submitted for 16S rRNA gene sequencing. Results: Genotype-based differences in microbial richness, structure, and composition were identified, but were not statistically significant. Taxonomic analysis revealed that the relative abundance of the phyla Fusobacteria and Bacteroidetes, and genus Fusobacterium were nominally increased in carriers compared to non-carriers, but were non-significant after correction for multiple testing. We also detected rare bacteria including Oligella that was reported only once in the middle ear. Conclusions: These findings suggest that A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome. Knowledge of middle ear microbial profiles according to genetic background can be potentially useful for therapeutic and prophylactic interventions for otitis media and can guide public health interventions towards decreasing otitis media prevalence within the indigenous Filipino community.

    KW - A2ML1

    KW - Indigenous population

    KW - Microbiome

    KW - Middle ear

    KW - Oligella

    KW - Otitis media

    KW - Philippines

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