Middle east respiratory syndrome coronavirus nsp1 inhibits host gene expression by selectively targeting mRNAs transcribed in the nucleus while sparing mRNAs of cytoplasmic origin

Kumari G. Lokugamage, Krishna Narayanan, Keisuke Nakagawa, Kaori Terasaki, Sydney I. Ramirez, Chien-Te Tseng, Shinji Makino

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome CoV (SARS-CoV) represent highly pathogenic human CoVs that share a property to inhibit host gene expression at the posttranscriptional level. Similar to the nonstructural protein 1 (nsp1) of SARS-CoV that inhibits host gene expression at the translational level, we report that MERS-CoV nsp1 also exhibits a conserved function to negatively regulate host gene expression by inhibiting host mRNA translation and inducing the degradation of host mRNAs. Furthermore, like SARS-CoV nsp1, the mRNA degradation activity of MERS-CoV nsp1, most probably triggered by its ability to induce an endonucleolytic RNA cleavage, was separable from its translation inhibitory function. Despite these functional similarities, MERS-CoV nsp1 used a strikingly different strategy that selectively targeted translationally competent host mRNAs for inhibition. While SARS-CoV nsp1 is localized exclusively in the cytoplasm and binds to the 40S ribosomal subunit to gain access to translating mRNAs, MERS-CoV nsp1 was distributed in both the nucleus and the cytoplasm and did not bind stably to the 40S subunit, suggesting a distinctly different mode of targeting translating mRNAs. Interestingly, consistent with this notion, MERS-CoV nsp1 selectively targeted mRNAs, which are transcribed in the nucleus and transported to the cytoplasm, for translation inhibition and mRNA degradation but spared exogenous mRNAs introduced directly into the cytoplasm or virus-like mRNAs that originate in the cytoplasm. Collectively, these data point toward a novel viral strategy wherein the cytoplasmic origin of MERS-CoV mRNAs facilitates their escape from the inhibitory effects of MERS-CoV nsp1.

Original languageEnglish (US)
Pages (from-to)10970-10981
Number of pages12
JournalJournal of Virology
Volume89
Issue number21
DOIs
StatePublished - 2015

Fingerprint

Gene Expression
Severe Acute Respiratory Syndrome
gene expression
Messenger RNA
Cytoplasm
cytoplasm
RNA Stability
proteins
translation (genetics)
degradation
Eukaryotic Small Ribosome Subunits
RNA Cleavage
Proteins
Middle East Respiratory Syndrome Coronavirus
coronavirus nonstructural protein
Middle East respiratory syndrome coronavirus
Protein Biosynthesis
Viruses
RNA
viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Middle east respiratory syndrome coronavirus nsp1 inhibits host gene expression by selectively targeting mRNAs transcribed in the nucleus while sparing mRNAs of cytoplasmic origin. / Lokugamage, Kumari G.; Narayanan, Krishna; Nakagawa, Keisuke; Terasaki, Kaori; Ramirez, Sydney I.; Tseng, Chien-Te; Makino, Shinji.

In: Journal of Virology, Vol. 89, No. 21, 2015, p. 10970-10981.

Research output: Contribution to journalArticle

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