Milestones in progression of primary pneumonic plague in cynomolgus macaques

Frederick Koster, David S. Perlin, Steven Park, Trevor Brasel, Andrew Gigliotti, Edward Barr, Leslie Myers, Robert C. Layton, Robert Sherwood, C. R. Lyons

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Vaccines against primary pneumonic plague, a potential bioweapon, must be tested for efficacy in well-characterized nonhuman primate models. Telemetered cynomolgus macaques (Macaca fascicularis) were challenged by the aerosol route with doses equivalent to approximately 100 50% effective doses of Yersinia pestis strain CO92 and necropsied at 24-h intervals postexposure (p.e.). Data for telemetered heart rates, respiratory rates, and increases in the temperature greater than the diurnal baseline values identified the onset of the systemic response at 55 to 60 h p.e. in all animals observed for at least 70 h p.e. Bacteremia was detected at 72 h p.e. by a Yersinia 16S rRNA-specific quantitative reverse transcription-PCR and was detected later by the culture method at the time of moribund necropsy. By 72 h p.e. multilobar pneumonia with diffuse septal inflammation consistent with early bacteremia was established, and all lung tissues had a high bacterial burden. The levels of cytokines or chemokines in serum were not significantly elevated at any time, and only the interleukin-1β, CCL2, and CCL3 levels were elevated in lung tissue. Inhalational plague in the cynomolgus macaque inoculated by the aerosol route produces most clinical features of the human disease, and in addition the disease progression mimics the disease progression from the anti-inflammatory phase to the proinflammatory phase described for the murine model. Defined milestones of disease progression, particularly the onset of fever, tachypnea, and bacteremia, should be useful for evaluating the efficacy of candidate vaccines.

Original languageEnglish
Pages (from-to)2946-2955
Number of pages10
JournalInfection and Immunity
Volume78
Issue number7
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Plague
Macaca
Bacteremia
Disease Progression
Aerosols
Vaccines
Yersinia pestis
Tachypnea
Yersinia
Lung
Macaca fascicularis
Respiratory Rate
Interleukin-1
Chemokines
Primates
Reverse Transcription
Pneumonia
Anti-Inflammatory Agents
Fever
Heart Rate

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Koster, F., Perlin, D. S., Park, S., Brasel, T., Gigliotti, A., Barr, E., ... Lyons, C. R. (2010). Milestones in progression of primary pneumonic plague in cynomolgus macaques. Infection and Immunity, 78(7), 2946-2955. https://doi.org/10.1128/IAI.01296-09

Milestones in progression of primary pneumonic plague in cynomolgus macaques. / Koster, Frederick; Perlin, David S.; Park, Steven; Brasel, Trevor; Gigliotti, Andrew; Barr, Edward; Myers, Leslie; Layton, Robert C.; Sherwood, Robert; Lyons, C. R.

In: Infection and Immunity, Vol. 78, No. 7, 07.2010, p. 2946-2955.

Research output: Contribution to journalArticle

Koster, F, Perlin, DS, Park, S, Brasel, T, Gigliotti, A, Barr, E, Myers, L, Layton, RC, Sherwood, R & Lyons, CR 2010, 'Milestones in progression of primary pneumonic plague in cynomolgus macaques', Infection and Immunity, vol. 78, no. 7, pp. 2946-2955. https://doi.org/10.1128/IAI.01296-09
Koster F, Perlin DS, Park S, Brasel T, Gigliotti A, Barr E et al. Milestones in progression of primary pneumonic plague in cynomolgus macaques. Infection and Immunity. 2010 Jul;78(7):2946-2955. https://doi.org/10.1128/IAI.01296-09
Koster, Frederick ; Perlin, David S. ; Park, Steven ; Brasel, Trevor ; Gigliotti, Andrew ; Barr, Edward ; Myers, Leslie ; Layton, Robert C. ; Sherwood, Robert ; Lyons, C. R. / Milestones in progression of primary pneumonic plague in cynomolgus macaques. In: Infection and Immunity. 2010 ; Vol. 78, No. 7. pp. 2946-2955.
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