Mineralocorticoid binding in cultured smooth muscle cells and fibroblasts from rat aorta

Walter J. Meyer, Nancy R. Nichols

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

Based on changes in electrolyte content, the direct action of adrenal steroids on blood vessel wall has been postulated for many years. High affinity corticosteroid binding sites have been found in both smooth muscle and fibroblast cultures from aortic explants of Sprague-Dawley rats. [3H]-aldosterone and [3H]-corticosterone bind to the same two distinct classes of sites: (a) corticoid receptor I with a Kd ranging from 0.11 to 1.31 × 10-9 M and a Bmax ranging from 22 to 282 mol × 10-18/μg DNA (measured by either steroid); (b) corticoid receptor II with a Kd ranging from 2.09 to 20.83 × 10-9 M and a Binmax ranging from 97 to 1410 mol × 10-18/μg DNA (measured by [3H]-aldostcronc) or a Bmax ranging from 1336 to 5520 mol × 10-18/μg DNA (measured by [3H]-corticosterone or [3H]-dexamethasone). Corticoid receptor I is easily detected by [3H]-aldostcrone in the presence of 40 nM dexamethasone and is not detected by [3H]-dexamethasone alone. Displacement experiments with corticoid receptor I labeled with [3H]-aldoslerone indicate the following hierarchy of binding: corticosterone ≥ desoxycorticosterone ≥ aldosterone > cortisol ≥ dexamethasone > 17β-estradiol = 5α-dihydrotestosterone. Displacement experiments with corticoid receptor II labeled with [3H]-dexamethasone indicate the following binding hierarchy: corticosterone = desoxycorticosterone = cortisol = dexamethasone > aldosterone > 17β-estradiol = 5α-dihydrotestosterone. The steroid specificity of corticoid receptor I is unique and distinguishes these sites from classical mineralocorticoid or glucocorticoid receptors. These sites may mediate new physiological effects of corticosterone, desoxycorticosterone and/or aldosterone in vascular tissue.

Original languageEnglish (US)
Pages (from-to)1157-1168
Number of pages12
JournalJournal of Steroid Biochemistry
Volume14
Issue number11
DOIs
StatePublished - Nov 1981

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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