Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart

Alfredo Saavedra-Molina, J. Ramírez-Emiliano, M. Clemente-Guerrero, V. Pérez-Vázquez, L. Aguilera-Aguirre, J. C. González-Hernández

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalAmino Acids
Volume24
Issue number1-2
StatePublished - Mar 2003
Externally publishedYes

Fingerprint

Rats
Mitochondria
Nitric Oxide
Adenosine Triphosphate
Calcium
Heart Mitochondria
Oxygen
Poisons
Divalent Cations
Oxygen Consumption
Free Radicals
Adenosine Triphosphatases
Arginine
Molecules

Keywords

  • ATP synthesis
  • Calcium
  • Heart
  • Hypoxia-reoxygenation
  • Mitochondria
  • Nitric oxide

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

Saavedra-Molina, A., Ramírez-Emiliano, J., Clemente-Guerrero, M., Pérez-Vázquez, V., Aguilera-Aguirre, L., & González-Hernández, J. C. (2003). Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart. Amino Acids, 24(1-2), 95-102.

Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart. / Saavedra-Molina, Alfredo; Ramírez-Emiliano, J.; Clemente-Guerrero, M.; Pérez-Vázquez, V.; Aguilera-Aguirre, L.; González-Hernández, J. C.

In: Amino Acids, Vol. 24, No. 1-2, 03.2003, p. 95-102.

Research output: Contribution to journalArticle

Saavedra-Molina, A, Ramírez-Emiliano, J, Clemente-Guerrero, M, Pérez-Vázquez, V, Aguilera-Aguirre, L & González-Hernández, JC 2003, 'Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart', Amino Acids, vol. 24, no. 1-2, pp. 95-102.
Saavedra-Molina A, Ramírez-Emiliano J, Clemente-Guerrero M, Pérez-Vázquez V, Aguilera-Aguirre L, González-Hernández JC. Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart. Amino Acids. 2003 Mar;24(1-2):95-102.
Saavedra-Molina, Alfredo ; Ramírez-Emiliano, J. ; Clemente-Guerrero, M. ; Pérez-Vázquez, V. ; Aguilera-Aguirre, L. ; González-Hernández, J. C. / Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart. In: Amino Acids. 2003 ; Vol. 24, No. 1-2. pp. 95-102.
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