Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart

Alfredo Saavedra-Molina; J. Ramírez-Emiliano; M. Clemente-Guerrero; V. Pérez-Vázquez; L. Aguilera-Aguirre; J. C. González-Hernández

doi:10.1007/s00726-002-0331-7

Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart

Alfredo Saavedra-Molina
, J. Ramírez-Emiliano
, M. Clemente-Guerrero
, V. Pérez-Vázquez
, L. Aguilera-Aguirre
, J. C. González-Hernández

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.

Original language	English (US)
Pages (from-to)	95-102
Number of pages	8
Journal	Amino Acids
Volume	24
Issue number	1-2
DOIs	https://doi.org/10.1007/s00726-002-0331-7
State	Published - Mar 2003
Externally published	Yes

Keywords

ATP synthesis
Calcium
Heart
Hypoxia-reoxygenation
Mitochondria
Nitric oxide

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/s00726-002-0331-7

Cite this

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title = "Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart",

abstract = "Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.",

keywords = "ATP synthesis, Calcium, Heart, Hypoxia-reoxygenation, Mitochondria, Nitric oxide",

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volume = "24",

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TY - JOUR

T1 - Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart

AU - Saavedra-Molina, Alfredo

AU - Ramírez-Emiliano, J.

AU - Clemente-Guerrero, M.

AU - Pérez-Vázquez, V.

AU - Aguilera-Aguirre, L.

AU - González-Hernández, J. C.

PY - 2003/3

Y1 - 2003/3

N2 - Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.

AB - Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.

KW - ATP synthesis

KW - Calcium

KW - Heart

KW - Hypoxia-reoxygenation

KW - Mitochondria

KW - Nitric oxide

UR - https://www.scopus.com/pages/publications/0037354568

UR - https://www.scopus.com/pages/publications/0037354568#tab=citedBy

U2 - 10.1007/s00726-002-0331-7

DO - 10.1007/s00726-002-0331-7

M3 - Article

C2 - 12624740

AN - SCOPUS:0037354568

SN - 0939-4451

VL - 24

SP - 95

EP - 102

JO - Amino Acids

JF - Amino Acids

IS - 1-2

ER -

Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart

Abstract

Keywords

ASJC Scopus subject areas

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