Mitochondrial thioredoxin in regulation of oxidant-induced cell death

Yan Chen, Jiyang Cai, Dean P. Jones

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposure to peroxides and diamide. Overexpression of mtTrx protected against diamide-induced oxidation and cytotoxicity. Oxidation of mtTrx was also achieved by knocking down its reductase; and lead to increased susceptibility to cell death. The data indicate that the redox status of mtTrx is a regulatory mechanism underlying the vulnerability of mitochondria to oxidative injury.

Original languageEnglish (US)
Pages (from-to)6596-6602
Number of pages7
JournalFEBS Letters
Volume580
Issue number28-29
DOIs
StatePublished - Dec 11 2006
Externally publishedYes

Fingerprint

Thioredoxins
Cell death
Oxidants
Cell Death
Diamide
Oxidation
Oxidation-Reduction
Mitochondria
Oxidative stress
Peroxides
Cytotoxicity
Oxidoreductases
Oxidative Stress
Association reactions
Apoptosis
Wounds and Injuries

Keywords

  • Mitochondria
  • Oxidative stress
  • Redox
  • Thioredoxin
  • Thioredoxin reductase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mitochondrial thioredoxin in regulation of oxidant-induced cell death. / Chen, Yan; Cai, Jiyang; Jones, Dean P.

In: FEBS Letters, Vol. 580, No. 28-29, 11.12.2006, p. 6596-6602.

Research output: Contribution to journalArticle

Chen, Yan ; Cai, Jiyang ; Jones, Dean P. / Mitochondrial thioredoxin in regulation of oxidant-induced cell death. In: FEBS Letters. 2006 ; Vol. 580, No. 28-29. pp. 6596-6602.
@article{d56d69754b294e668b0778dce6833071,
title = "Mitochondrial thioredoxin in regulation of oxidant-induced cell death",
abstract = "Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposure to peroxides and diamide. Overexpression of mtTrx protected against diamide-induced oxidation and cytotoxicity. Oxidation of mtTrx was also achieved by knocking down its reductase; and lead to increased susceptibility to cell death. The data indicate that the redox status of mtTrx is a regulatory mechanism underlying the vulnerability of mitochondria to oxidative injury.",
keywords = "Mitochondria, Oxidative stress, Redox, Thioredoxin, Thioredoxin reductase",
author = "Yan Chen and Jiyang Cai and Jones, {Dean P.}",
year = "2006",
month = "12",
day = "11",
doi = "10.1016/j.febslet.2006.11.007",
language = "English (US)",
volume = "580",
pages = "6596--6602",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "28-29",

}

TY - JOUR

T1 - Mitochondrial thioredoxin in regulation of oxidant-induced cell death

AU - Chen, Yan

AU - Cai, Jiyang

AU - Jones, Dean P.

PY - 2006/12/11

Y1 - 2006/12/11

N2 - Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposure to peroxides and diamide. Overexpression of mtTrx protected against diamide-induced oxidation and cytotoxicity. Oxidation of mtTrx was also achieved by knocking down its reductase; and lead to increased susceptibility to cell death. The data indicate that the redox status of mtTrx is a regulatory mechanism underlying the vulnerability of mitochondria to oxidative injury.

AB - Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposure to peroxides and diamide. Overexpression of mtTrx protected against diamide-induced oxidation and cytotoxicity. Oxidation of mtTrx was also achieved by knocking down its reductase; and lead to increased susceptibility to cell death. The data indicate that the redox status of mtTrx is a regulatory mechanism underlying the vulnerability of mitochondria to oxidative injury.

KW - Mitochondria

KW - Oxidative stress

KW - Redox

KW - Thioredoxin

KW - Thioredoxin reductase

UR - http://www.scopus.com/inward/record.url?scp=33751509841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751509841&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.11.007

DO - 10.1016/j.febslet.2006.11.007

M3 - Article

C2 - 17113580

AN - SCOPUS:33751509841

VL - 580

SP - 6596

EP - 6602

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 28-29

ER -