Mobility of Histidine Side Chains Analyzed with 15 N NMR Relaxation and Cross-Correlation Data

Insight into Zinc-Finger-DNA Interactions

Catherine A. Kemme, Ross H. Luu, Chuanying Chen, Channing C. Pletka, Bernard Pettitt, Junji Iwahara

Research output: Contribution to journalArticle

Abstract

Due to chemical exchange, the mobility of histidine (His) side chains of proteins is typically difficult to analyze by NMR spectroscopy. Using an NMR approach that is uninfluenced by chemical exchange, we investigated internal motions of the His imidazole NH groups that directly interact with DNA phosphates in the Egr-1 zinc-finger-DNA complex. In this approach, the transverse and longitudinal cross-correlation rates for 15 N chemical shift anisotropy and 15 N- 1 H dipole-dipole relaxation interference were analyzed together with 15 N longitudinal relaxation rates and heteronuclear Overhauser effect data at two magnetic field strengths. We found that the zinc-coordinating His side chains directly interacting with DNA phosphates are strongly restricted in mobility. This makes a contrast to the arginine and lysine side chains that retain high mobility despite their interactions with DNA phosphates in the same complex. The entropic effects of side-chain mobility on the molecular association are discussed.

Original languageEnglish (US)
Pages (from-to)3706-3710
Number of pages5
JournalJournal of Physical Chemistry B
Volume123
Issue number17
DOIs
StatePublished - May 2 2019

Fingerprint

histidine
Zinc Fingers
Histidine
cross correlation
Zinc
DNA
deoxyribonucleic acid
zinc
Nuclear magnetic resonance
phosphates
nuclear magnetic resonance
Phosphates
interactions
dipoles
Overhauser effect
Arginine
lysine
Anisotropy
Chemical shift
Magnetic Fields

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Mobility of Histidine Side Chains Analyzed with 15 N NMR Relaxation and Cross-Correlation Data : Insight into Zinc-Finger-DNA Interactions. / Kemme, Catherine A.; Luu, Ross H.; Chen, Chuanying; Pletka, Channing C.; Pettitt, Bernard; Iwahara, Junji.

In: Journal of Physical Chemistry B, Vol. 123, No. 17, 02.05.2019, p. 3706-3710.

Research output: Contribution to journalArticle

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AB - Due to chemical exchange, the mobility of histidine (His) side chains of proteins is typically difficult to analyze by NMR spectroscopy. Using an NMR approach that is uninfluenced by chemical exchange, we investigated internal motions of the His imidazole NH groups that directly interact with DNA phosphates in the Egr-1 zinc-finger-DNA complex. In this approach, the transverse and longitudinal cross-correlation rates for 15 N chemical shift anisotropy and 15 N- 1 H dipole-dipole relaxation interference were analyzed together with 15 N longitudinal relaxation rates and heteronuclear Overhauser effect data at two magnetic field strengths. We found that the zinc-coordinating His side chains directly interacting with DNA phosphates are strongly restricted in mobility. This makes a contrast to the arginine and lysine side chains that retain high mobility despite their interactions with DNA phosphates in the same complex. The entropic effects of side-chain mobility on the molecular association are discussed.

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