Modeling placental transport

Correlation of in vitro BeWo cell permeability and ex vivo human placental perfusion

Marie Sønnegaard Poulsen, Erik Rytting, Tina Mose, Lisbeth E. Knudsen

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The placental passage of three compounds with different physicochemical properties was recently investigated in ex vivo human placental perfusion experiments (caffeine, benzoic acid, and glyphosate) [Mose, T., Kjaerstad, M.B., Mathiesen, L., Nielsen, J.B., Edelfors, S., Knudsen, L.E., 2008. Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. J. Toxicol. Environ. Health, Part A 71, 984-991]. In this work, the transport of these same three compounds, plus the reference compound antipyrine, was investigated using BeWo (b30) cell monolayers. Transport across the BeWo cells was observed in the rank order of caffeine > antipyrine > benzoic acid > glyphosate in terms of both the apparent permeability coefficient and the initial slope, defined as the linear rate of substance transferred to the fetal compartment as percent per time, a parameter used to compare the two experimental models. The results from the in vitro studies were in excellent agreement with the ex vivo results (caffeine ≈ antipyrine > benzoic acid > glyphosate). However the transfer rate was much slower in the BeWo cells compared to the perfusion system. The advantages and limitations of each model are discussed in order to assist in the preparation, prediction, and performance of future studies of maternal-fetal transfer.

Original languageEnglish (US)
Pages (from-to)1380-1386
Number of pages7
JournalToxicology in Vitro
Volume23
Issue number7
DOIs
StatePublished - Oct 2009
Externally publishedYes

Fingerprint

glyphosate
Benzoic Acid
Caffeine
Antipyrine
Permeability
Perfusion
Hydraulic conductivity
Monolayers
Theoretical Models
Mothers
Health
In Vitro Techniques

Keywords

  • Benzoic acid
  • BeWo cells
  • Caffeine
  • Glyphosate
  • Modelling
  • Placental transport

ASJC Scopus subject areas

  • Toxicology

Cite this

Modeling placental transport : Correlation of in vitro BeWo cell permeability and ex vivo human placental perfusion. / Poulsen, Marie Sønnegaard; Rytting, Erik; Mose, Tina; Knudsen, Lisbeth E.

In: Toxicology in Vitro, Vol. 23, No. 7, 10.2009, p. 1380-1386.

Research output: Contribution to journalArticle

Poulsen, Marie Sønnegaard ; Rytting, Erik ; Mose, Tina ; Knudsen, Lisbeth E. / Modeling placental transport : Correlation of in vitro BeWo cell permeability and ex vivo human placental perfusion. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 7. pp. 1380-1386.
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