Modification of endothelial cell functions by hantaan virus infection

Prolonged hyper-permeability induced by TNF-alplia of hantaan virus-infected endothelial cell monolayers

M. Niikura, A. Maeda, Tetsuro Ikegami, M. Saijo, I. Kurane, S. Morikawa

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Serious vascular leakage is central to the pathogenesis of hantavirus infections. However, there is no evidence suggesting the hantavirus infection of endothelial cells directly causes obvious cell damage or morphological alteration either in vivo or in vitro. In this study, we examined whether Hantaan virus (HTNV) infection modifies the barrier function of endothelial cell monolayers upon the exposure to pro-inflammatory cytokines. Low levels (1 ng/ml) of tumor necrosis factor-alpha initially increased the permeability in both HTNV-infected and uninfected monolayers similarly. Thereafter, however, these monolayers showed significant difference. The HTNV-infected monolayers remained irreversibly hyper-permeable during the experimental period up to 4 days, while the uninfected monolayers completely recovered the barrier function. The prolonged hyperpermeability of HTNV-infected monolayers was not associated with cell death or gap formation in the monolayers, and was independent from their nitric oxide or prostaglandin production. These results are the first evidence that hantavirus infection modifies barrier function of endothelial cell monolayers and suggest that HTNV-infection of endothelial cells may contribute to the increased vascular leakage through the prolonged response to cytokines.

Original languageEnglish (US)
Pages (from-to)1279-1292
Number of pages14
JournalArchives of Virology
Volume149
Issue number7
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Hantaan virus
Virus Diseases
Hantavirus Infections
Permeability
Endothelial Cells
Blood Vessels
Cytokines
Prostaglandins
Nitric Oxide
Cell Death
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Modification of endothelial cell functions by hantaan virus infection : Prolonged hyper-permeability induced by TNF-alplia of hantaan virus-infected endothelial cell monolayers. / Niikura, M.; Maeda, A.; Ikegami, Tetsuro; Saijo, M.; Kurane, I.; Morikawa, S.

In: Archives of Virology, Vol. 149, No. 7, 07.2004, p. 1279-1292.

Research output: Contribution to journalArticle

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