Modification of energy metabolism and radiation response of a murine tumor by changes in nitric oxide availability

P. J. Wood, I. J. Stratford, G. E. Adams, C. Szabo, C. Thiemermann, J. R. Vane

    Research output: Contribution to journalArticlepeer-review

    62 Scopus citations

    Abstract

    The nitric oxide donor, SIN-1 and nitric oxide synthase inhibitor, nitro-L-arginine were examined for their effects on energy metabolism using 31P magnetic resonance spectroscopy, and on radiation sensitivity in the transplantable murine tumour, SCCVII/Ha. SIN-1 at 2 mg/kg i.v. reduced Pi/total by 40-50% within 10 min and increased X-ray sensitivity 3 fold, consistent with increased tumour oxygenation. Nitro-L-arginine at 10 mg/kg i.v. increased Pi/total by 250% at 45 min and was maintained for at least 2 hr. Nitro-L-arginine also increased radiation resistance 3-5 fold, consistent with the induction of tumour hypoxia. The results indicate that tumour energy metabolism may be altered through drug induced modification of nitric oxide availability, and that these changes are sufficient to modify tumour sensitivity to X-rays.

    Original languageEnglish (US)
    Pages (from-to)505-510
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume192
    Issue number2
    DOIs
    StatePublished - Apr 30 1993

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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