Modification of metabolism of transplantable and spontaneous murine tumors by the nitric oxide synthase inhibitor, nitro-L-arginine

Pauline J. Wood, Janet M. Sansom, Ian J. Stratford, Gerald E. Adams, Csaba Szabo, Christoph Thiemermann, John R. Vane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose: To determine the effects of the nitric oxide synthase inhibitor, nitro-L-arginine on energy metabolism in transplantable and spontaneous murine tumors. Methods and Materials: The responses of the transplantable murine tumor SCCVII/Ha and a range of spontaneously arising murine mammary adenocarcinomas to 10 mg/kg IV nitro-L-arginine were examined using in vivo31P magnetic resonance spectroscopy (MRS). The influence of Hypnorm/Hypnovel anesthesia on the response to nitro-L-arginine was also determined in the SCCVII/Ha tumors. Data were expressed as changes in the inorganic phosphate peak area relative to the sum of all peak areas from the 31P MR spectrum, or Pi/total. Results: Nitro-L-arginine at 10 mg/kg IV increased Pi/total 2-3-fold in the SCCVII/Ha tumors for at least 2 h after administration, in both anesthetized and nonanesthetized mice, consistent with increased tumor hypoxia. Similar increases in Pi/total were observed after 10 mg/kg IV nitro-L-arginine in 13 spontaneous murine tumors from three different mouse strains, where anesthetic was used. Conclusion: The results indicate that tumor metabolism may be modified by an inhibitor of nitric oxide synthesis, that this modification occurs in both transplantable and spontaneous murine tumors and is not affected by anesthetic.

Original languageEnglish (US)
Pages (from-to)443-447
Number of pages5
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume29
Issue number3
DOIs
StatePublished - Jun 15 1994
Externally publishedYes

Fingerprint

metabolism
nitric oxide
Nitric Oxide Synthase
inhibitors
Arginine
tumors
Neoplasms
anesthetics
Anesthetics
mice
anesthesia
hypoxia
magnetic resonance spectroscopy
Energy Metabolism
Nitric Oxide
Adenocarcinoma
Breast
Magnetic Resonance Spectroscopy
Anesthesia
Phosphates

Keywords

  • P magnetic resonance spectroscopy
  • Nitric oxide synthase
  • Nitro-L-arginine
  • Spontaneous tumors
  • Tranplantable tumors

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Modification of metabolism of transplantable and spontaneous murine tumors by the nitric oxide synthase inhibitor, nitro-L-arginine. / Wood, Pauline J.; Sansom, Janet M.; Stratford, Ian J.; Adams, Gerald E.; Szabo, Csaba; Thiemermann, Christoph; Vane, John R.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 29, No. 3, 15.06.1994, p. 443-447.

Research output: Contribution to journalArticle

Wood, Pauline J. ; Sansom, Janet M. ; Stratford, Ian J. ; Adams, Gerald E. ; Szabo, Csaba ; Thiemermann, Christoph ; Vane, John R. / Modification of metabolism of transplantable and spontaneous murine tumors by the nitric oxide synthase inhibitor, nitro-L-arginine. In: International Journal of Radiation Oncology, Biology, Physics. 1994 ; Vol. 29, No. 3. pp. 443-447.
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