Modification of primary and recurrent genital herpes in guinea pigs by passive immunization

Nigel Bourne, Richard Pyles, David I. Bernstein, Lawrence R. Stanberry

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Guinea pigs were administered antiserum 24 h (As+24) or 72 h (As+72) after intravaginal herpes simplex virus type 2 (HSV-2) challenge. Treatment at either time reduced acute virus replication in the dorsal root ganglia and the overall magnitude of replication in the genital tract. In two studies, As+24 treatment significantly reduced the severity of primary genital skin disease and the frequency of subsequent spontaneous recurrent disease. In contrast, As+72 treatment produced a modest reduction in primary disease severity but did not impact on recurrent disease. Quantitative PCR analysis of dorsal root ganglia DNA from latently infected animals showed that As+24 treatment produced a significantly reduced viral DNA burden, which appeared to correlate with the reduction in recurrent disease. The amount of DNA in the ganglia of As+72-treated animals was not significantly lower than that of controls. These observations have implications for both the dynamics of latency establishment and desirable vaccine characteristics.

Original languageEnglish (US)
Pages (from-to)2797-2801
Number of pages5
JournalJournal of General Virology
Volume83
Issue number11
StatePublished - Nov 1 2002

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Herpes Genitalis
Passive Immunization
Guinea Pigs
Spinal Ganglia
Human Herpesvirus 2
DNA
Viral DNA
Therapeutics
Virus Replication
Viral Load
Skin Diseases
Ganglia
Immune Sera
Vaccines
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Modification of primary and recurrent genital herpes in guinea pigs by passive immunization. / Bourne, Nigel; Pyles, Richard; Bernstein, David I.; Stanberry, Lawrence R.

In: Journal of General Virology, Vol. 83, No. 11, 01.11.2002, p. 2797-2801.

Research output: Contribution to journalArticle

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