Modification of primary and recurrent genital herpes in guinea pigs by passive immunization

Nigel Bourne, Richard B. Pyles, David I. Bernstein, Lawrence R. Stanberry

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Guinea pigs were administered antiserum 24 h (As+24) or 72 h (As+72) after intravaginal herpes simplex virus type 2 (HSV-2) challenge. Treatment at either time reduced acute virus replication in the dorsal root ganglia and the overall magnitude of replication in the genital tract. In two studies, As+24 treatment significantly reduced the severity of primary genital skin disease and the frequency of subsequent spontaneous recurrent disease. In contrast, As+72 treatment produced a modest reduction in primary disease severity but did not impact on recurrent disease. Quantitative PCR analysis of dorsal root ganglia DNA from latently infected animals showed that As+24 treatment produced a significantly reduced viral DNA burden, which appeared to correlate with the reduction in recurrent disease. The amount of DNA in the ganglia of As+72-treated animals was not significantly lower than that of controls. These observations have implications for both the dynamics of latency establishment and desirable vaccine characteristics.

Original languageEnglish (US)
Pages (from-to)2797-2801
Number of pages5
JournalJournal of General Virology
Volume83
Issue number11
DOIs
StatePublished - Nov 1 2002

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ASJC Scopus subject areas

  • Virology

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