Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons

Qing Yang, Zi Zhen Wu, Xiao Li, Zhi Wang Li, Jin Bo Wei, Qi Sheng Hu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The modulatory effect of oxytocin (OT) on ATP-activated currents (IATP) was studied in freshly isolated dorsal root ganglion (DRG) neurons of rats using whole cell clamp technique. In most of the neurons examined (50/70, 71.4%) extracellular application of OT (10-9-10-5 mol/L) suppressed IATP while in the rest (20/70, 28.6%) no modulatory effect was observed. OT shifted the ATP concentration-response curve downwards with a decrease of 39.8±4.2% in the maximal current response and with no significant change of Kd value. This OT-induced inhibition of IATP showed no voltage dependence, and could be blocked by [d(CH2)5,Tyr(Me)2,Thr4,Tyr-NH 29]-OVT (d(CH2)5-OVT) (10-8 mol/L), a specific OT receptor antagonist. Intracellular application of H-9 (4×10-5 mol/L, an inhibitor of protein kinase A) (n=12), BAPTA (10-2 mol/L, a chelator of calcium ions) (n=4) could reverse the inhibitory effect of extracellular OT (10-7 mol), while inclusion of H-7 (2×10-5 mol/L, a protein kinase C inhibitior) (n=8) and KN-93 (10-5 mol/L, an inhibitor of CaMKII) (n=9) in the recording pipette did not affect this effect. The results suggested that OT inhibition on ATP-activated currents was mediated by OT receptors in the membrane of DRG neurons; and this inhibitory effect involved the transduction of intracellular cAMP-PKA and Ca2+.

Original languageEnglish (US)
Pages (from-to)910-916
Number of pages7
JournalNeuropharmacology
Volume43
Issue number5
DOIs
StatePublished - Oct 2002
Externally publishedYes

Fingerprint

Spinal Ganglia
Oxytocin
Adenosine Triphosphate
Neurons
Oxytocin Receptors
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
Ions
Membranes

Keywords

  • ATP
  • Dorsal root ganglion
  • Intracellular dialysis
  • Oxytocin
  • Whole cell recording

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons. / Yang, Qing; Wu, Zi Zhen; Li, Xiao; Li, Zhi Wang; Wei, Jin Bo; Hu, Qi Sheng.

In: Neuropharmacology, Vol. 43, No. 5, 10.2002, p. 910-916.

Research output: Contribution to journalArticle

Yang, Qing ; Wu, Zi Zhen ; Li, Xiao ; Li, Zhi Wang ; Wei, Jin Bo ; Hu, Qi Sheng. / Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons. In: Neuropharmacology. 2002 ; Vol. 43, No. 5. pp. 910-916.
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