Modulation of glycine receptor chloride channels by cAMP-dependent protein kinase in spinal trigeminal neurons

Yumin Song, Li-Yen Huang

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

GLYCINE is an important inhibitory transmitter in the brainstem and spinal cord1,2. In the trigeminal subnucleus caudalis (medullary dorsal horn) and in the spinal dorsal horn (the relaying centres for processing pain and sensory information3-5), glycine inhibits the glutamate-evoked depolarization and depresses firing of neurons6. The binding of glycine to its receptor produces a large increase in Cl- conductance, which causes membrane hyperpolarization7. The selectivity and gating properties of glycine receptor channels have been well characterized8; the glycine receptor molecules have also been purified9-11. The amino-acid sequence, deduced from complementary DNA clones encoding one of the peptides (the 48K subunit)12, shows significant homology with γ-aminobutyric acid A (GABAA) and nicotinic acetylcholine receptor subunits, suggesting that glycine receptors may belong to a superfamily of chemically gated channel proteins12-13. However, very little is known about the modulation of glycine receptor channels. We have investigated the regulation of strychnine-sensitive glycine receptor channels by cyclic AMP-dependent protein kinase in neurons isolated from spinal trigeminal nucleus of rat and report here that the protein kinase A dramatically increased the glycine-induced Cl- currents by increasing the probability of the channel openings. Gs protein, which is sensitive to cholera toxin, was involved in the modulation.

Original languageEnglish (US)
Pages (from-to)242-245
Number of pages4
JournalNature
Volume348
Issue number6298
StatePublished - Nov 15 1990

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Glycine Receptors
Chloride Channels
Cyclic AMP-Dependent Protein Kinases
Neurons
Glycine
Spinal Trigeminal Nucleus
Aminobutyrates
Pain Clinics
Strychnine
Cholera Toxin
Nicotinic Receptors
Brain Stem
Glutamic Acid
Amino Acid Sequence
Complementary DNA
Clone Cells
Peptides
Membranes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Modulation of glycine receptor chloride channels by cAMP-dependent protein kinase in spinal trigeminal neurons. / Song, Yumin; Huang, Li-Yen.

In: Nature, Vol. 348, No. 6298, 15.11.1990, p. 242-245.

Research output: Contribution to journalArticle

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