Modulation of human basophil migration in vitro by a soluble factor from virus-stimulated leukocytes

Michael A. Lett-Brown; John J. Hooks; Jerzy A. Georgides; David O. Thueson; J. Andrew Grant

doi:10.1016/0090-1229(81)90176-8

Modulation of human basophil migration in vitro by a soluble factor from virus-stimulated leukocytes

Michael A. Lett-Brown, John J. Hooks, Jerzy A. Georgides, David O. Thueson, J. Andrew Grant

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Although it is clear that respiratory virus infections frequently provoke attacks of asthma, the mechanism of the provocation is unknown. The accumulation of basophils in the sputum and the presence of basophil chemotactic factors in the serum of allergic patients suggests that the basophil may play a causative role in asthmatic attacks. We have investigated the effect of viruses on the in vitro migration of human basophils. When peripheral blood leukocytes from normal subjects were preincubated for 24 hr with uv-inactivated Sendai virus, the chemotactic response of basophils was augmented. This effect was also seen with the cell-free supernatant of mononuclear cells cultured with virus. Our results suggest that the soluble product mediating the enhancement is interferon. While the soluble factor enhanced basophil migration toward two chemoattractants (C5 peptide and a synthetic formyl-tripeptide), it has no effect on the migration of monocytes.

Original language	English (US)
Pages (from-to)	179-187
Number of pages	9
Journal	Clinical Immunology and Immunopathology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/0090-1229(81)90176-8
State	Published - Aug 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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10.1016/0090-1229(81)90176-8

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title = "Modulation of human basophil migration in vitro by a soluble factor from virus-stimulated leukocytes",

abstract = "Although it is clear that respiratory virus infections frequently provoke attacks of asthma, the mechanism of the provocation is unknown. The accumulation of basophils in the sputum and the presence of basophil chemotactic factors in the serum of allergic patients suggests that the basophil may play a causative role in asthmatic attacks. We have investigated the effect of viruses on the in vitro migration of human basophils. When peripheral blood leukocytes from normal subjects were preincubated for 24 hr with uv-inactivated Sendai virus, the chemotactic response of basophils was augmented. This effect was also seen with the cell-free supernatant of mononuclear cells cultured with virus. Our results suggest that the soluble product mediating the enhancement is interferon. While the soluble factor enhanced basophil migration toward two chemoattractants (C5 peptide and a synthetic formyl-tripeptide), it has no effect on the migration of monocytes.",

author = "Lett-Brown, {Michael A.} and Hooks, {John J.} and Georgides, {Jerzy A.} and Thueson, {David O.} and Grant, {J. Andrew}",

note = "Funding Information: Dr. Thueson is the recipient of a Young Investigator Award AI-14879 and Dr. Grant of an Allergic Diseases Academic Award AI-001 10, both from the National Institute of Allergy and Infectious Diseases. This project was initiated while Dr. Lett-Brown was a Visiting Postdoctoral Fellow at the National Institute of Dental Research (NIDR). The authors would like to thank Dr. Siraganian (NIDR) for his support, Ms. M. Aelvoet for her technical assistance, and Ms. Mary Babcock for excellent secretarial assistance. This work was presented in part at the National Meeting of the American Society for Clinical Investigation, Washington, D.C., May 1980, and was published in abstract form in Clinical Research 28, 505A. The study was supported in part by Research Grant Al-12621 from the National Institute of Allergy and Infectious Diseases.",

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AU - Grant, J. Andrew

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N2 - Although it is clear that respiratory virus infections frequently provoke attacks of asthma, the mechanism of the provocation is unknown. The accumulation of basophils in the sputum and the presence of basophil chemotactic factors in the serum of allergic patients suggests that the basophil may play a causative role in asthmatic attacks. We have investigated the effect of viruses on the in vitro migration of human basophils. When peripheral blood leukocytes from normal subjects were preincubated for 24 hr with uv-inactivated Sendai virus, the chemotactic response of basophils was augmented. This effect was also seen with the cell-free supernatant of mononuclear cells cultured with virus. Our results suggest that the soluble product mediating the enhancement is interferon. While the soluble factor enhanced basophil migration toward two chemoattractants (C5 peptide and a synthetic formyl-tripeptide), it has no effect on the migration of monocytes.

AB - Although it is clear that respiratory virus infections frequently provoke attacks of asthma, the mechanism of the provocation is unknown. The accumulation of basophils in the sputum and the presence of basophil chemotactic factors in the serum of allergic patients suggests that the basophil may play a causative role in asthmatic attacks. We have investigated the effect of viruses on the in vitro migration of human basophils. When peripheral blood leukocytes from normal subjects were preincubated for 24 hr with uv-inactivated Sendai virus, the chemotactic response of basophils was augmented. This effect was also seen with the cell-free supernatant of mononuclear cells cultured with virus. Our results suggest that the soluble product mediating the enhancement is interferon. While the soluble factor enhanced basophil migration toward two chemoattractants (C5 peptide and a synthetic formyl-tripeptide), it has no effect on the migration of monocytes.

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Modulation of human basophil migration in vitro by a soluble factor from virus-stimulated leukocytes

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