Modulation of [3H]dihydropyridine receptors by activation of protein kinase C in chick muscle cells

Research output: Contribution to journalArticle

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Abstract

Modulation of ion channels is an essential step for understanding the regulation of cellular functions. 1,4-Dihydropyridines (nitrendepine, nifedipine, PN 200-110, etc.) are potent inhibitors of voltage-dependent calcium channels and are important therapeutic agents in the treatment of various cardiovascular disorders such as angina and cardiac arrhythmias. In this work a new procedure is employed to determine the density of surface dihydropyridine receptors in contracting muscle cells in culture. Activation of endogenous protein kinase C (the Ca2+/phospholipid-dependent enzyme) by the tumor promoter phorbol-12-myristate or 1-oleoyl-2-acetylglycerol enhanced the number of dihydropyridine receptors without significant change in the receptor affinity. The increase in the number of receptors was associated with stimulation of the dihydropyridine-sensitive 45Ca uptake as well as activation of protein kinase C in myotubes treated with phorbol esters. These data strongly suggest that activation of protein kinase C promotes the appearance of dihydropyridine receptors in the plasma membrane.

Original languageEnglish (US)
Pages (from-to)4649-4652
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number10
StatePublished - 1987
Externally publishedYes

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L-Type Calcium Channels
Muscle Cells
Protein Kinase C
Muscle
Chemical activation
Cells
Modulation
Isradipine
Skeletal Muscle Fibers
Phorbol Esters
Calcium Channels
Cell membranes
Nifedipine
Ion Channels
Cell culture
Carcinogens
Cardiac Arrhythmias
Phospholipids
Cell Culture Techniques
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Modulation of [3H]dihydropyridine receptors by activation of protein kinase C in chick muscle cells. / Navarro, Javier.

In: Journal of Biological Chemistry, Vol. 262, No. 10, 1987, p. 4649-4652.

Research output: Contribution to journalArticle

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