Modulation of superoxide production of alveolar macrophages and peripheral blood mononuclear cells by β-agonists and theophylline

William Calhoun, C. A. Stevens, S. B. Lambert

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Reactive oxygen species, including superoxide anion, have attracted increasing attention for their possible role in promoting inflammation in a variety of pulmonary diseases including asthma. However, reactive oxygen species metabolism of phagocytic cells may be substantially modified by therapeutic agents used for asthma. Peripheral blood mononuclear cells and alveolar macrophages from 15 normal subjects, and blood mononuclear cells from an additional 17 normal subjects, were studied to assess the effects of β-receptor agonists and theophylline on phagocytic cell superoxide release. Isoproterenol produced a biphasic effect on spontaneous and phorbol ester stimulated alveolar macrophage and mononuclear cell superoxide production, augmenting release at 10-5M, and inhibiting release at 10-4M. These effects on spontaneous function in mononuclear cells were inhibited by 10-5M propranolol. Under conditions of phorbol ester-stimulation the enhancing effect of 10-5M isoproterenol on blood mononuclear cells was blocked by propranolol, but the inhibitory effect of 10-4M isoproterenol was not. Albuterol at equimolar concentrations with isoproterenol was not associated with altered spontaneous or stimulated superoxide release by alveolar macrophages or mononuclear cells. Further, spontaneous superoxide release by alveolar macrophages and mononuclear cells was significantly reduced by therapeutically achievable concentrations of theophylline (>5μg/ml). We conclude that the medication history must be controlled in studies of cell function in asthma, and that albuterol may be preferable to isoproterenol as a premedication for bronchoscopy when superoxide production of airspace cells is studied.

Original languageEnglish (US)
Pages (from-to)514-522
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume117
Issue number6
StatePublished - 1991
Externally publishedYes

Fingerprint

Alveolar Macrophages
Theophylline
Superoxides
Blood Cells
Isoproterenol
Blood
Modulation
Asthma
Albuterol
Phorbol Esters
Phagocytes
Propranolol
Reactive Oxygen Species
Pulmonary diseases
Premedication
Bronchoscopy
Metabolism
Lung Diseases
Inflammation

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Modulation of superoxide production of alveolar macrophages and peripheral blood mononuclear cells by β-agonists and theophylline. / Calhoun, William; Stevens, C. A.; Lambert, S. B.

In: Journal of Laboratory and Clinical Medicine, Vol. 117, No. 6, 1991, p. 514-522.

Research output: Contribution to journalArticle

@article{f1598751de0040d0bde502c20254e027,
title = "Modulation of superoxide production of alveolar macrophages and peripheral blood mononuclear cells by β-agonists and theophylline",
abstract = "Reactive oxygen species, including superoxide anion, have attracted increasing attention for their possible role in promoting inflammation in a variety of pulmonary diseases including asthma. However, reactive oxygen species metabolism of phagocytic cells may be substantially modified by therapeutic agents used for asthma. Peripheral blood mononuclear cells and alveolar macrophages from 15 normal subjects, and blood mononuclear cells from an additional 17 normal subjects, were studied to assess the effects of β-receptor agonists and theophylline on phagocytic cell superoxide release. Isoproterenol produced a biphasic effect on spontaneous and phorbol ester stimulated alveolar macrophage and mononuclear cell superoxide production, augmenting release at 10-5M, and inhibiting release at 10-4M. These effects on spontaneous function in mononuclear cells were inhibited by 10-5M propranolol. Under conditions of phorbol ester-stimulation the enhancing effect of 10-5M isoproterenol on blood mononuclear cells was blocked by propranolol, but the inhibitory effect of 10-4M isoproterenol was not. Albuterol at equimolar concentrations with isoproterenol was not associated with altered spontaneous or stimulated superoxide release by alveolar macrophages or mononuclear cells. Further, spontaneous superoxide release by alveolar macrophages and mononuclear cells was significantly reduced by therapeutically achievable concentrations of theophylline (>5μg/ml). We conclude that the medication history must be controlled in studies of cell function in asthma, and that albuterol may be preferable to isoproterenol as a premedication for bronchoscopy when superoxide production of airspace cells is studied.",
author = "William Calhoun and Stevens, {C. A.} and Lambert, {S. B.}",
year = "1991",
language = "English (US)",
volume = "117",
pages = "514--522",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Modulation of superoxide production of alveolar macrophages and peripheral blood mononuclear cells by β-agonists and theophylline

AU - Calhoun, William

AU - Stevens, C. A.

AU - Lambert, S. B.

PY - 1991

Y1 - 1991

N2 - Reactive oxygen species, including superoxide anion, have attracted increasing attention for their possible role in promoting inflammation in a variety of pulmonary diseases including asthma. However, reactive oxygen species metabolism of phagocytic cells may be substantially modified by therapeutic agents used for asthma. Peripheral blood mononuclear cells and alveolar macrophages from 15 normal subjects, and blood mononuclear cells from an additional 17 normal subjects, were studied to assess the effects of β-receptor agonists and theophylline on phagocytic cell superoxide release. Isoproterenol produced a biphasic effect on spontaneous and phorbol ester stimulated alveolar macrophage and mononuclear cell superoxide production, augmenting release at 10-5M, and inhibiting release at 10-4M. These effects on spontaneous function in mononuclear cells were inhibited by 10-5M propranolol. Under conditions of phorbol ester-stimulation the enhancing effect of 10-5M isoproterenol on blood mononuclear cells was blocked by propranolol, but the inhibitory effect of 10-4M isoproterenol was not. Albuterol at equimolar concentrations with isoproterenol was not associated with altered spontaneous or stimulated superoxide release by alveolar macrophages or mononuclear cells. Further, spontaneous superoxide release by alveolar macrophages and mononuclear cells was significantly reduced by therapeutically achievable concentrations of theophylline (>5μg/ml). We conclude that the medication history must be controlled in studies of cell function in asthma, and that albuterol may be preferable to isoproterenol as a premedication for bronchoscopy when superoxide production of airspace cells is studied.

AB - Reactive oxygen species, including superoxide anion, have attracted increasing attention for their possible role in promoting inflammation in a variety of pulmonary diseases including asthma. However, reactive oxygen species metabolism of phagocytic cells may be substantially modified by therapeutic agents used for asthma. Peripheral blood mononuclear cells and alveolar macrophages from 15 normal subjects, and blood mononuclear cells from an additional 17 normal subjects, were studied to assess the effects of β-receptor agonists and theophylline on phagocytic cell superoxide release. Isoproterenol produced a biphasic effect on spontaneous and phorbol ester stimulated alveolar macrophage and mononuclear cell superoxide production, augmenting release at 10-5M, and inhibiting release at 10-4M. These effects on spontaneous function in mononuclear cells were inhibited by 10-5M propranolol. Under conditions of phorbol ester-stimulation the enhancing effect of 10-5M isoproterenol on blood mononuclear cells was blocked by propranolol, but the inhibitory effect of 10-4M isoproterenol was not. Albuterol at equimolar concentrations with isoproterenol was not associated with altered spontaneous or stimulated superoxide release by alveolar macrophages or mononuclear cells. Further, spontaneous superoxide release by alveolar macrophages and mononuclear cells was significantly reduced by therapeutically achievable concentrations of theophylline (>5μg/ml). We conclude that the medication history must be controlled in studies of cell function in asthma, and that albuterol may be preferable to isoproterenol as a premedication for bronchoscopy when superoxide production of airspace cells is studied.

UR - http://www.scopus.com/inward/record.url?scp=0025830986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025830986&partnerID=8YFLogxK

M3 - Article

VL - 117

SP - 514

EP - 522

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 6

ER -