Modulation of tendon healing by nitric oxide

G. A C Murrell, Csaba Szabo, J. A. Hannafin, D. Jang, M. M. Dolan, X. H. Deng, D. F. Murrell, R. F. Warren

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Nitric oxide (NO.) is a small, diffusible free radical that is generated from L-arginine by a family of enzymes, collectively termed the nitric oxide synthases. We investigated the role of NO. in tendon healing. NO. synthase activity and immunoreactivity was absent in un-injured rat Achilles tendon. After surgical division there was a five-fold increase in NO. synthase activity and immunoreactivity within the healing tendon at day 7, with a return to near baseline levels at day 14. Inhibition of NO. synthase activity with oral administration of Nω-nitro-L-arginine methyl ester (L-NAME) resulted in a significant reduction in cross-sectional area (30% at day 7, p < 0.01, 50% at day 15, p < 0.001) and failure load (24% at day 7, p < 0.01) of the healing Achilles tendon constructs. Rats fed the same regimen of the enantiomer of L-NAME, (D-NAME) had normal tendon healing. These results indicate that nitric oxide synthase is induced during tendon healing and inhibition of nitric oxide synthase inhibits this tendon healing.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalInflammation Research
Volume46
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Tendons
Nitric Oxide
Modulation
Nitric Oxide Synthase
Achilles Tendon
NG-Nitroarginine Methyl Ester
Rats
Free Radicals
Oral Administration
Arginine
Enantiomers
Esters
Enzymes

Keywords

  • Free radical
  • Nitric oxide
  • Nitric oxide synthase
  • Tendon healing
  • Wound healing

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Murrell, G. A. C., Szabo, C., Hannafin, J. A., Jang, D., Dolan, M. M., Deng, X. H., ... Warren, R. F. (1997). Modulation of tendon healing by nitric oxide. Inflammation Research, 46(1), 19-27. https://doi.org/10.1007/s000110050027

Modulation of tendon healing by nitric oxide. / Murrell, G. A C; Szabo, Csaba; Hannafin, J. A.; Jang, D.; Dolan, M. M.; Deng, X. H.; Murrell, D. F.; Warren, R. F.

In: Inflammation Research, Vol. 46, No. 1, 1997, p. 19-27.

Research output: Contribution to journalArticle

Murrell, GAC, Szabo, C, Hannafin, JA, Jang, D, Dolan, MM, Deng, XH, Murrell, DF & Warren, RF 1997, 'Modulation of tendon healing by nitric oxide', Inflammation Research, vol. 46, no. 1, pp. 19-27. https://doi.org/10.1007/s000110050027
Murrell GAC, Szabo C, Hannafin JA, Jang D, Dolan MM, Deng XH et al. Modulation of tendon healing by nitric oxide. Inflammation Research. 1997;46(1):19-27. https://doi.org/10.1007/s000110050027
Murrell, G. A C ; Szabo, Csaba ; Hannafin, J. A. ; Jang, D. ; Dolan, M. M. ; Deng, X. H. ; Murrell, D. F. ; Warren, R. F. / Modulation of tendon healing by nitric oxide. In: Inflammation Research. 1997 ; Vol. 46, No. 1. pp. 19-27.
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