Modulation of the frequency of human cytomegalovirus-induced chromosome aberrations by camptothecin

Cheng Zong Deng, Sazaly AbuBakar, Michael P. Fons, Istvan Boldogh, Thomas Albrecht

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The effects of selected DNA repair inhibitors on the frequency of human cytomegalovirus (HCMV)-induced chromosome aberrations were evaluated in human peripheral blood lymphocytes (PBLs). Treatment of HCMV-infected PBLs with camptothecin (0.05 to 0.3 μg/ml), an inhibitor of topoisomerase I, for 30 hr resulted in a significant (P < 0.01) synergistic enhancement of the frequency of HCMV-induced chromosome damage. On the other hand, a significant increase in the frequency of chromosome damage was not noted for infected PBLs treated with either 3-aminobenzamide (3-AB; 3 to 30 μg/ml), an inhibitor of poly(ADP-ribose) polymerase, or novobiocin (3 to 30 μg/ml), an inhibitor of topoisomerase II or excision repair processes, for 30 hr. Chromatid-type breaks and exchanges were the predominant type of chromosome aberrations observed in the HCMV-infected cells treated with camptothecin, suggesting that HCMV infection is associated with the induction of single-strand DNA breaks. Furthermore, these findings suggest that HCMV infection does not inflict direct DNA damage which is repaired through 3-AB- or novobiocin-sensitive pathways.

Original languageEnglish (US)
Pages (from-to)397-401
Number of pages5
JournalVirology
Volume189
Issue number1
DOIs
StatePublished - Jul 1992

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Modulation of the frequency of human cytomegalovirus-induced chromosome aberrations by camptothecin'. Together they form a unique fingerprint.

  • Cite this