Modulation of the frequency of human cytomegalovirus-induced chromosome aberrations by camptothecin

Cheng Zong Deng, Sazaly AbuBakar, Michael P. Fons, Istvan Boldogh, Thomas Albrecht

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effects of selected DNA repair inhibitors on the frequency of human cytomegalovirus (HCMV)-induced chromosome aberrations were evaluated in human peripheral blood lymphocytes (PBLs). Treatment of HCMV-infected PBLs with camptothecin (0.05 to 0.3 μg/ml), an inhibitor of topoisomerase I, for 30 hr resulted in a significant (P < 0.01) synergistic enhancement of the frequency of HCMV-induced chromosome damage. On the other hand, a significant increase in the frequency of chromosome damage was not noted for infected PBLs treated with either 3-aminobenzamide (3-AB; 3 to 30 μg/ml), an inhibitor of poly(ADP-ribose) polymerase, or novobiocin (3 to 30 μg/ml), an inhibitor of topoisomerase II or excision repair processes, for 30 hr. Chromatid-type breaks and exchanges were the predominant type of chromosome aberrations observed in the HCMV-infected cells treated with camptothecin, suggesting that HCMV infection is associated with the induction of single-strand DNA breaks. Furthermore, these findings suggest that HCMV infection does not inflict direct DNA damage which is repaired through 3-AB- or novobiocin-sensitive pathways.

Original languageEnglish (US)
Pages (from-to)397-401
Number of pages5
JournalVirology
Volume189
Issue number1
DOIs
StatePublished - 1992

Fingerprint

Camptothecin
Cytomegalovirus
Chromosome Aberrations
Novobiocin
Cytomegalovirus Infections
Lymphocytes
DNA Repair
Chromosomes
Topoisomerase I Inhibitors
Topoisomerase II Inhibitors
Single-Stranded DNA Breaks
Chromatids
DNA Damage

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Modulation of the frequency of human cytomegalovirus-induced chromosome aberrations by camptothecin. / Deng, Cheng Zong; AbuBakar, Sazaly; Fons, Michael P.; Boldogh, Istvan; Albrecht, Thomas.

In: Virology, Vol. 189, No. 1, 1992, p. 397-401.

Research output: Contribution to journalArticle

Deng, Cheng Zong ; AbuBakar, Sazaly ; Fons, Michael P. ; Boldogh, Istvan ; Albrecht, Thomas. / Modulation of the frequency of human cytomegalovirus-induced chromosome aberrations by camptothecin. In: Virology. 1992 ; Vol. 189, No. 1. pp. 397-401.
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