Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase

Xiaochun Jin, Nemat Morsy, John Winston, Pankaj J. Pasricha, Kennon Garrett, Hamid I. Akbarali

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The capsaicin receptor TRPV1 is a nonselective cation channel that is expressed in sensory neurons. In this study, we examined the role of the nonreceptor cellular tyrosine kinase c-Src kinase in the modulation of the rat TRPV1. Capsaicin-induced currents in identified colonic dorsal root ganglion neurons were blocked by the c-Src kinase inhibitor PP2 and enhanced by the tyrosine phosphatase inhibitor sodium orthovandate. PP2 also abolished currents inhuman embryonic kidney-293 cells transfected with rat TRPV1, whereas cotransfection of TRPV1 with v-Src resulted in fivefold increase in capsaicin-induced currents. In cells transfected with dominant-negative c-Src and TRPV 1, capsaicin-induced currents were decreased by approximately fourfold. TRPV1 co-immunoprecipitated with Src kinase and was tyrosine phosphorylated. These studies demonstrate that TRPV1 is a potential target for cellular tyrosine kinase-dependent phosphorylation.

Original languageEnglish (US)
Pages (from-to)C558-C563
JournalAmerican Journal of Physiology - Cell Physiology
Volume287
Issue number2 56-2
DOIs
StatePublished - Aug 2004

Keywords

  • Capsaicin
  • Cation channel
  • Immunoprecipitation
  • Inflammatory bowel disease
  • Pain
  • Pp

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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