Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase

Xiaochun Jin, Nemat Morsy, John Winston, Pankaj J. Pasricha, Kennon Garrett, Hamid I. Akbarali

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The capsaicin receptor TRPV1 is a nonselective cation channel that is expressed in sensory neurons. In this study, we examined the role of the nonreceptor cellular tyrosine kinase c-Src kinase in the modulation of the rat TRPV1. Capsaicin-induced currents in identified colonic dorsal root ganglion neurons were blocked by the c-Src kinase inhibitor PP2 and enhanced by the tyrosine phosphatase inhibitor sodium orthovandate. PP2 also abolished currents inhuman embryonic kidney-293 cells transfected with rat TRPV1, whereas cotransfection of TRPV1 with v-Src resulted in fivefold increase in capsaicin-induced currents. In cells transfected with dominant-negative c-Src and TRPV 1, capsaicin-induced currents were decreased by approximately fourfold. TRPV1 co-immunoprecipitated with Src kinase and was tyrosine phosphorylated. These studies demonstrate that TRPV1 is a potential target for cellular tyrosine kinase-dependent phosphorylation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume287
Issue number2 56-2
DOIs
StatePublished - Aug 2004

Fingerprint

Capsaicin
Induced currents
Protein-Tyrosine Kinases
Modulation
Neurons
Tyrosine
Rats
TRPV Cation Channels
Phosphorylation
src-Family Kinases
Spinal Ganglia
Sensory Receptor Cells
Phosphoric Monoester Hydrolases
Cations
Sodium
Kidney
CSK tyrosine-protein kinase

Keywords

  • Capsaicin
  • Cation channel
  • Immunoprecipitation
  • Inflammatory bowel disease
  • Pain
  • Pp

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase. / Jin, Xiaochun; Morsy, Nemat; Winston, John; Pasricha, Pankaj J.; Garrett, Kennon; Akbarali, Hamid I.

In: American Journal of Physiology - Cell Physiology, Vol. 287, No. 2 56-2, 08.2004.

Research output: Contribution to journalArticle

Jin, Xiaochun ; Morsy, Nemat ; Winston, John ; Pasricha, Pankaj J. ; Garrett, Kennon ; Akbarali, Hamid I. / Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase. In: American Journal of Physiology - Cell Physiology. 2004 ; Vol. 287, No. 2 56-2.
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