Molecular and clinical evidence of Ehrlichia chaffeensis infection in Cameroonian patients with undifferentiated febrile illness

L. M. Ndip, M. Labruna, R. N. Ndip, David Walker, Jere McBride

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In the U.S.A., human monocytotropic ehrlichiosis (HME) caused by Ehrlichia chaffeensis is an emerging ticktransmitted zoonosis. In Cameroon, where E. canis, E. chaffeensis and E. ewingii have recently been detected in dogs and/or ticks (Rhipicephalus sanguineus), the potential exists for human infections. Patients from the coastal region of Cameroon who had acute fevers of unknown aetiology were therefore checked for ehrlichial infection, using a realtime PCR that amplifies part of a genus-specific gene (dsb) that codes for a disulphide-bond formation protein. Ehrlichial blood was detected in the peripheral blood from 12 (10%) of the 118 patients investigated by PCR. When the 12 amplicons from the positive cases were sequenced, they were found to be identical to each other and to the corresponding dsb sequence of an Arkansas strain of E. chaffeensis. The 12 patients who were PCR-positive for E. chaffeensis suffered from fever (100%), headache (67%), myalgia (42%), arthralgia (58%), pulmonary involvement (17%) and/or a diffuse rash (17%).

Original languageEnglish (US)
Pages (from-to)719-725
Number of pages7
JournalAnnals of Tropical Medicine and Parasitology
Volume103
Issue number8
DOIs
StatePublished - Dec 2009

Fingerprint

Ehrlichia chaffeensis
Fever
Cameroon
Infection
Polymerase Chain Reaction
Rhipicephalus sanguineus
Ehrlichiosis
Myalgia
Zoonoses
Arthralgia
Ticks
Exanthema
Disulfides
Headache
Dogs
Lung
Genes
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Molecular and clinical evidence of Ehrlichia chaffeensis infection in Cameroonian patients with undifferentiated febrile illness. / Ndip, L. M.; Labruna, M.; Ndip, R. N.; Walker, David; McBride, Jere.

In: Annals of Tropical Medicine and Parasitology, Vol. 103, No. 8, 12.2009, p. 719-725.

Research output: Contribution to journalArticle

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