Molecular approaches to the role of the microbiota in inflammatory bowel disease

Charles O. Elson, Yingzi Cong, Fengxia Qi, Robert M. Hershberg, Stephan R. Targan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

The microbiota plays a crucial role in experimental models of inflammatory bowel disease, but the exact mechanisms of its effects are unknown. These studies took two molecular approaches to this question. The first used amplification of the 16s ribosomal DNA to define microbial diversity in the colon. Although there were differences in colitic and non-colitic mice, we could not determine whether this was primary or secondary to the disease. The second approach used serologic expression cloning to identify the microbial proteins stimulating the pathogenic immune response. Previously unknown microbial flagellins were the dominant cluster of antigens identified. About half of the sera from patients with Crohn's disease have IgG antibodies to these flagellins.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages39-51
Number of pages13
Volume1072
DOIs
StatePublished - 2006
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1072
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Flagellin
Microbiota
Inflammatory Bowel Diseases
Ribosomal DNA
Crohn Disease
Organism Cloning
Colon
Theoretical Models
Immunoglobulin G
Cloning
Antigens
Amplification
Antibodies
Serum
Proteins

Keywords

  • Antibodies
  • Colitis
  • Flagellin
  • Microbiota
  • Ribosomal DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Elson, C. O., Cong, Y., Qi, F., Hershberg, R. M., & Targan, S. R. (2006). Molecular approaches to the role of the microbiota in inflammatory bowel disease. In Annals of the New York Academy of Sciences (Vol. 1072, pp. 39-51). (Annals of the New York Academy of Sciences; Vol. 1072). https://doi.org/10.1196/annals.1326.010

Molecular approaches to the role of the microbiota in inflammatory bowel disease. / Elson, Charles O.; Cong, Yingzi; Qi, Fengxia; Hershberg, Robert M.; Targan, Stephan R.

Annals of the New York Academy of Sciences. Vol. 1072 2006. p. 39-51 (Annals of the New York Academy of Sciences; Vol. 1072).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Elson, CO, Cong, Y, Qi, F, Hershberg, RM & Targan, SR 2006, Molecular approaches to the role of the microbiota in inflammatory bowel disease. in Annals of the New York Academy of Sciences. vol. 1072, Annals of the New York Academy of Sciences, vol. 1072, pp. 39-51. https://doi.org/10.1196/annals.1326.010
Elson CO, Cong Y, Qi F, Hershberg RM, Targan SR. Molecular approaches to the role of the microbiota in inflammatory bowel disease. In Annals of the New York Academy of Sciences. Vol. 1072. 2006. p. 39-51. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1326.010
Elson, Charles O. ; Cong, Yingzi ; Qi, Fengxia ; Hershberg, Robert M. ; Targan, Stephan R. / Molecular approaches to the role of the microbiota in inflammatory bowel disease. Annals of the New York Academy of Sciences. Vol. 1072 2006. pp. 39-51 (Annals of the New York Academy of Sciences).
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