Molecular biology: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA

Catherine L. Jopling, Min Kyung Yi, Alissa M. Lancaster, Stanley M. Lemon, Peter Sarnow

Research output: Contribution to journalArticle

1856 Scopus citations

Abstract

MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5′ noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.

Original languageEnglish (US)
Pages (from-to)1577-1581
Number of pages5
JournalScience
Volume309
Issue number5740
DOIs
StatePublished - Sep 2 2005

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