Molecular biology: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA

Catherine L. Jopling; Min Kyung Yi; Alissa M. Lancaster; Stanley M. Lemon; Peter Sarnow

doi:10.1126/science.1113329

Molecular biology: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA

Catherine L. Jopling
, Min Kyung Yi
, Alissa M. Lancaster
, Stanley M. Lemon
, Peter Sarnow

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

2287 Scopus citations

Abstract

MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5′ noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.

Original language	English (US)
Pages (from-to)	1577-1581
Number of pages	5
Journal	Science
Volume	309
Issue number	5740
DOIs	https://doi.org/10.1126/science.1113329
State	Published - Sep 2 2005

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title = "Molecular biology: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA",

abstract = "MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5′ noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.",

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AU - Jopling, Catherine L.

AU - Yi, Min Kyung

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AU - Lemon, Stanley M.

AU - Sarnow, Peter

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AB - MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5′ noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.

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Molecular biology: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA

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