Molecular characterization and antimicrobial susceptibility of nasal staphylococcus aureus isolates from a chinese medical college campus

Jimei Du, Chun Chen, Baixing Ding, Jinjing Tu, Zhiqiang Qin, Chris Parsons, Cassandra Salgado, Qiangjun Cai, Yulong Song, Qiyu Bao, Liming Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu

Research output: Contribution to journalArticle

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Abstract

Staphylococcus aureus colonization and infection occur more commonly among persons living or working in crowded conditions, but characterization of S. aureus colonization within medical communities in China is lacking. A total of 144 (15.4%, 144/935) S. aureus isolates, including 28 (3.0%, 28/935) MRSA isolates, were recovered from the nares of 935 healthy human volunteers residing on a Chinese medical college campus. All S. aureus isolates were susceptible to vancomycin, quinupristin/dalfopristin and linezolid but the majority were resistant to penicillin (96.5%), ampicillin/sulbactam (83.3%) and trimethoprim/sulfamethoxazole (93.1%). 82%, (23/28) of the MRSA isolates and 66% (77/116) of the MSSA isolates were resistant to multiple antibiotics, and 3 MRSA isolates were resistant to mupirocin-an agent commonly used for nasal decolonization. 16 different sequence types (STs), as well as SCCmec genes II, III, IVd, and V, were represented among MRSA isolates. We also identified, for the first time, two novel STs (ST1778 and ST1779) and 5 novel spa types for MRSA. MRSA isolates were distributed in different sporadic clones, and ST59-MRSA-VId- t437 was found within 3 MRSA isolates. Moreover, one isolate with multidrug resistance belonging to ST398-MRSA-V- t571 associated with animal infections was identified, and 3 isolates distributed in three different clones harbored PVL genes. Collectively, these data indicate a high prevalence of nasal MRSA carriage and molecular heterogeneity of S. aureus isolates among persons residing on a Chinese medical college campus. Identification of epidemic MRSA clones associated with community infection supports the need for more effective infection control measures to reduce nasal carriage and prevent dissemination of MRSA to hospitalized patients and health care workers in this community.

Original languageEnglish (US)
Article numbere27328
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 17 2011
Externally publishedYes

Fingerprint

Linezolid
Methicillin-Resistant Staphylococcus aureus
Nose
Staphylococcus aureus
Genes
anti-infective agents
Mupirocin
Sulfamethoxazole Drug Combination Trimethoprim
Vancomycin
Health care
Penicillins
clones
Animals
Anti-Bacterial Agents
sulbactam
infection
sulfamethoxazole
patient care
vancomycin
multiple drug resistance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Molecular characterization and antimicrobial susceptibility of nasal staphylococcus aureus isolates from a chinese medical college campus. / Du, Jimei; Chen, Chun; Ding, Baixing; Tu, Jinjing; Qin, Zhiqiang; Parsons, Chris; Salgado, Cassandra; Cai, Qiangjun; Song, Yulong; Bao, Qiyu; Zhang, Liming; Pan, Jingye; Wang, Liangxing; Yu, Fangyou.

In: PLoS One, Vol. 6, No. 11, e27328, 17.11.2011.

Research output: Contribution to journalArticle

Du, J, Chen, C, Ding, B, Tu, J, Qin, Z, Parsons, C, Salgado, C, Cai, Q, Song, Y, Bao, Q, Zhang, L, Pan, J, Wang, L & Yu, F 2011, 'Molecular characterization and antimicrobial susceptibility of nasal staphylococcus aureus isolates from a chinese medical college campus', PLoS One, vol. 6, no. 11, e27328. https://doi.org/10.1371/journal.pone.0027328
Du, Jimei ; Chen, Chun ; Ding, Baixing ; Tu, Jinjing ; Qin, Zhiqiang ; Parsons, Chris ; Salgado, Cassandra ; Cai, Qiangjun ; Song, Yulong ; Bao, Qiyu ; Zhang, Liming ; Pan, Jingye ; Wang, Liangxing ; Yu, Fangyou. / Molecular characterization and antimicrobial susceptibility of nasal staphylococcus aureus isolates from a chinese medical college campus. In: PLoS One. 2011 ; Vol. 6, No. 11.
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