Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India

Mehuli Sarkar, Anurodh S. Agrawal, Rakhi Sharma Dey, Shiladitya Chattopadhyay, Ranajoy Mullick, Papiya De, Sekhar Chakrabarti, Mamta Chawla-Sarkar

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino acid sequences of pH1N1 strains revealed HA1 and NS1 to be of North American swine lineage, and the NA gene of Eurasian swine lineage. Consistent with previous reports, the PB1-F2 gene of pH1N1 strains was unique due to a mutation resulting in a truncated protein of 11 aa. The HA, NA and NS1 genes of H1N1/2009 strains clustered with H1N1 strains of 2000-2009, whereas a subset of strains contained a pH1N1-like truncated PB1-F2. The truncated PB1-F2 may confer the advantage of lower pathogenicity but higher replication and infectivity to the human H1N1 strains. This is the first report of seasonal H1N1/2009 strains with a pH1N1/2009-like gene segment.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalArchives of virology
Volume156
Issue number2
DOIs
StatePublished - Feb 1 2011

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India'. Together they form a unique fingerprint.

  • Cite this

    Sarkar, M., Agrawal, A. S., Dey, R. S., Chattopadhyay, S., Mullick, R., De, P., Chakrabarti, S., & Chawla-Sarkar, M. (2011). Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India. Archives of virology, 156(2), 207-217. https://doi.org/10.1007/s00705-010-0842-6