TY - JOUR
T1 - Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India
AU - Sarkar, Mehuli
AU - Agrawal, Anurodh S.
AU - Dey, Rakhi Sharma
AU - Chattopadhyay, Shiladitya
AU - Mullick, Ranajoy
AU - De, Papiya
AU - Chakrabarti, Sekhar
AU - Chawla-Sarkar, Mamta
N1 - Funding Information:
This study was supported by financial assistance from the Indian Council of Medical Research (ICMR), Department of Health Research, New Delhi. M. Sarkar is supported by Women Scientist Scheme-A, Department of Science and Technology, Govt of India. Dr. R. Sharma Dey and A. S. Agrawal are supported by SRA- Pool Scheme, CSIR and Senior Research Fellowship from ICMR, respectively. The authors acknowledge technical support by Swati Ghosh, Malay Dey-Sarkar, Shyam Jaiswara and Shyamal Mandal. The authors are thankful for the support provided by the Administration, Store/Purchase and Accounts departments during the pandemic period.
PY - 2011/2
Y1 - 2011/2
N2 - During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino acid sequences of pH1N1 strains revealed HA1 and NS1 to be of North American swine lineage, and the NA gene of Eurasian swine lineage. Consistent with previous reports, the PB1-F2 gene of pH1N1 strains was unique due to a mutation resulting in a truncated protein of 11 aa. The HA, NA and NS1 genes of H1N1/2009 strains clustered with H1N1 strains of 2000-2009, whereas a subset of strains contained a pH1N1-like truncated PB1-F2. The truncated PB1-F2 may confer the advantage of lower pathogenicity but higher replication and infectivity to the human H1N1 strains. This is the first report of seasonal H1N1/2009 strains with a pH1N1/2009-like gene segment.
AB - During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino acid sequences of pH1N1 strains revealed HA1 and NS1 to be of North American swine lineage, and the NA gene of Eurasian swine lineage. Consistent with previous reports, the PB1-F2 gene of pH1N1 strains was unique due to a mutation resulting in a truncated protein of 11 aa. The HA, NA and NS1 genes of H1N1/2009 strains clustered with H1N1 strains of 2000-2009, whereas a subset of strains contained a pH1N1-like truncated PB1-F2. The truncated PB1-F2 may confer the advantage of lower pathogenicity but higher replication and infectivity to the human H1N1 strains. This is the first report of seasonal H1N1/2009 strains with a pH1N1/2009-like gene segment.
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U2 - 10.1007/s00705-010-0842-6
DO - 10.1007/s00705-010-0842-6
M3 - Article
C2 - 20981560
AN - SCOPUS:79551573073
SN - 0304-8608
VL - 156
SP - 207
EP - 217
JO - Archives of virology
JF - Archives of virology
IS - 2
ER -