Molecular characterization of a novel Ljungan virus (Parechovirus; Picornaviridae) reveals a fourth genotype and indicates ancestral recombination

Conny Tolf, Maria Gullberg, E. Susanne Johansson, Robert B. Tesh, Björn Andersson, A. Michael Lindberg

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ljungan virus (LV) was discovered 20 years ago in Swedish bank voles (Myodes glareolus, previously referred to as Clethrionomys glareolus) during the search for an infectious agent causing lethal myocarditis in young athletes. To date, the genomes of four LV isolates, including the prototype 87-012 strain, have been characterized. Three of these LV strains were isolated from bank voles trapped in Sweden. Sequence analysis of an American virus (M1146), isolated from a montane vole (Microtus montanus) in western USA, indicates that this strain represents a genotype that is different from the Swedish strains. Here, we present genomic analyses of a fifth LV strain (64-7855) isolated from a southern red-backed vole (Myodes gapperi) trapped during arbovirus studies in New York state in the north-eastern USA in the 1960s. Sequence analysis of the 64-7855 genome showed an LV-like genome organization and sequence similarity to other LV strains. Genetic and phylogenetic analyses of the evolutionary relationship between the 64-7855 strain and other viruses within the family Picornaviridae, including previously published LV strains, demonstrated that the 64-7855 strain constitutes a new genotype within the LV species. Analyses also showed that different regions of the 64-7855 genome have different phylogenetic relationships with other LV strains, indicating that previous recombination events have been involved in the evolution of this virus.

Original languageEnglish (US)
Pages (from-to)843-853
Number of pages11
JournalJournal of General Virology
Volume90
Issue number4
DOIs
StatePublished - 2009

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Parechovirus
Picornaviridae
Arvicolinae
Genetic Recombination
Genotype
Genome
Viruses
Sequence Analysis
Arboviruses
Myocarditis
Sweden
Athletes

ASJC Scopus subject areas

  • Virology

Cite this

Molecular characterization of a novel Ljungan virus (Parechovirus; Picornaviridae) reveals a fourth genotype and indicates ancestral recombination. / Tolf, Conny; Gullberg, Maria; Johansson, E. Susanne; Tesh, Robert B.; Andersson, Björn; Lindberg, A. Michael.

In: Journal of General Virology, Vol. 90, No. 4, 2009, p. 843-853.

Research output: Contribution to journalArticle

Tolf, Conny ; Gullberg, Maria ; Johansson, E. Susanne ; Tesh, Robert B. ; Andersson, Björn ; Lindberg, A. Michael. / Molecular characterization of a novel Ljungan virus (Parechovirus; Picornaviridae) reveals a fourth genotype and indicates ancestral recombination. In: Journal of General Virology. 2009 ; Vol. 90, No. 4. pp. 843-853.
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