Molecular characterization of cDNA for phospholipase A2-activating protein

Ashok Chopra, D. A. Ribardo, Thomas Wood, D. J. Prusak, X. J. Xu, Johnny Peterson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A phospholipase A2-activating protein (PLAP) cDNA was cloned and sequenced from a human monocyte cDNA library, and expressed as a histidine- tagged fusion protein. The DNA-deduced aa sequence of human PLAP was 80,826 Da; however, SDS-PAGE analysis revealed a 72-74 kDa protein which matched the size of native PLAP from human monocytes. Anti-sense plap oligonucleotide blocked cholera toxin-induced release of 3H-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A2 activity.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1444
Issue number1
DOIs
StatePublished - Jan 18 1999

Fingerprint

Complementary DNA
Monocytes
Antisense Oligonucleotides
Cholera Toxin
Phospholipases A2
Gene Library
Histidine
Arachidonic Acid
Polyacrylamide Gel Electrophoresis
Proteins
Fusion reactions
phospholipase A2-activating protein
DNA

Keywords

  • 3'-Rapid amplification of cDNA ends
  • Anti-sense plap oligonucleotide
  • CDNA cloning sequencing, and expression
  • Phospholipase A-activating protein
  • Phospholipase A-activating protein cDNA
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular characterization of cDNA for phospholipase A2-activating protein. / Chopra, Ashok; Ribardo, D. A.; Wood, Thomas; Prusak, D. J.; Xu, X. J.; Peterson, Johnny.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1444, No. 1, 18.01.1999, p. 125-130.

Research output: Contribution to journalArticle

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