Molecular characterization of extended-spectrum β-lactamase-producer klebsiella pneumoniae isolates causing neonatal sepsis in Peru

Coralith García, Lizeth Astocondor, Beatriz Rojo-Bezares, Jan Jacobs, Yolanda Sáenz

Research output: Contribution to journalArticle

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Abstract

Klebsiella pneumoniae (KP) is the most common cause of neonatal sepsis in the low-and middle-income countries. Our objective was to describe the phenotypic and molecular characteristics of extended-spectrum â-lactamase (ESBL)-producer KP in neonatal care centers from Peru. We collected 176 non-duplicate consecutive KP isolates from blood isolates of neonates from eight general public hospitals of Lima, Peru. The overall rate of ESBL production was 73.3% (N = 129). The resistance rates were higher among ESBL-producer isolates when compared with the nonproducers: 85.3% versus 12.8% for gentamicin (P <0.01), 59.7% versus 8.5% for trimethoprim-sulfamethoxazole (P <0.01), 45.0% versus 8.5% for ciprofloxacin (P <0.01), and 36.4% versus 12.8% for amikacin (P <0.01). A total of 359 â-lactamase-encoding genes were detected among 129 ESBL-producer isolates; 109 isolates (84.5%) carried two or more genes. Among 37 ESBL-producer isolates randomly selected, CTX-M-15 and CTX-M-2 were the most common ESBLs detected. Most of the isolates (92%) belonged to the group KpI. Pulsed-field gel electrophoresis showed that multiple KP clones were circulating among the eight neonatal units included.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume94
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Peru
Klebsiella pneumoniae
Amikacin
Pulsed Field Gel Electrophoresis
Public Hospitals
Sulfamethoxazole Drug Combination Trimethoprim
Ciprofloxacin
Gentamicins
General Hospitals
Genes
Clone Cells
Newborn Infant
Neonatal Sepsis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Molecular characterization of extended-spectrum β-lactamase-producer klebsiella pneumoniae isolates causing neonatal sepsis in Peru. / García, Coralith; Astocondor, Lizeth; Rojo-Bezares, Beatriz; Jacobs, Jan; Sáenz, Yolanda.

In: American Journal of Tropical Medicine and Hygiene, Vol. 94, No. 2, 01.02.2016, p. 285-288.

Research output: Contribution to journalArticle

García, Coralith ; Astocondor, Lizeth ; Rojo-Bezares, Beatriz ; Jacobs, Jan ; Sáenz, Yolanda. / Molecular characterization of extended-spectrum β-lactamase-producer klebsiella pneumoniae isolates causing neonatal sepsis in Peru. In: American Journal of Tropical Medicine and Hygiene. 2016 ; Vol. 94, No. 2. pp. 285-288.
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