Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008

Ming Hua Li, Shi Hong Fu, Yun Feng, Xiao Yan Gao, You Gang Zhai, De Shan Yu, Guo Tai Li, Yu Xin Jia, Guo Dong Liang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

OBJECTIVE: To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene. METHODS: Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4. RESULTS: The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain. CONCLUSION: Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.

Original languageEnglish (US)
Pages (from-to)251-253
Number of pages3
JournalZhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
Volume23
Issue number4
StatePublished - Aug 2009
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine

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