Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage

Emilio Garcia, Patrick Chain, Jeff M. Elliott, Alexander G. Bobrov, Vladimir Motin, Olga Kirillina, Victoria Lao, Richard Calendar, Andrey A. Filippov

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Our analysis of the plague diagnostic phage L-413C genome sequence and structure reveals that L-413C is highly similar and collinear with enterobacteriophage P2, though important differences were found. Of special interest was the mosaic nature of the tail fiber protein H in L-413C, given the differentiating specificity of this phage for Yersinia pestis vs. Yersinia pseudotuberculosis. While the N-terminal 207 and C-terminal 137 amino acids of L-413C display significant homology with the P2 H protein, a large (465 amino acid) middle section appears to be derived from a T4-related H protein, with highest similarity to the T6 and RB32 distal tail fibers. This finding along with appropriate preadsorption experiments suggest that the unique H protein of L-413C may be responsible for the specificity of this phage for Y. pestis, and that the Y. pestis receptors that are recognized and bound by L-413C either do not exist in Y. pseudotuberculosis or have a different structure.

Original languageEnglish (US)
Pages (from-to)85-96
Number of pages12
JournalVirology
Volume372
Issue number1
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Plague
Yersinia pestis
Bacteriophages
Yersinia pseudotuberculosis
Proteins
Amino Acids
Genome

Keywords

  • Genome sequencing
  • H protein
  • Host-specificity
  • Plague diagnostic bacteriophage
  • Preadsorbtion test
  • Tail fiber
  • Yersinia pestis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Garcia, E., Chain, P., Elliott, J. M., Bobrov, A. G., Motin, V., Kirillina, O., ... Filippov, A. A. (2008). Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage. Virology, 372(1), 85-96. https://doi.org/10.1016/j.virol.2007.10.032

Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage. / Garcia, Emilio; Chain, Patrick; Elliott, Jeff M.; Bobrov, Alexander G.; Motin, Vladimir; Kirillina, Olga; Lao, Victoria; Calendar, Richard; Filippov, Andrey A.

In: Virology, Vol. 372, No. 1, 01.03.2008, p. 85-96.

Research output: Contribution to journalArticle

Garcia, E, Chain, P, Elliott, JM, Bobrov, AG, Motin, V, Kirillina, O, Lao, V, Calendar, R & Filippov, AA 2008, 'Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage', Virology, vol. 372, no. 1, pp. 85-96. https://doi.org/10.1016/j.virol.2007.10.032
Garcia, Emilio ; Chain, Patrick ; Elliott, Jeff M. ; Bobrov, Alexander G. ; Motin, Vladimir ; Kirillina, Olga ; Lao, Victoria ; Calendar, Richard ; Filippov, Andrey A. / Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage. In: Virology. 2008 ; Vol. 372, No. 1. pp. 85-96.
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