Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage

Emilio Garcia, Patrick Chain, Jeff M. Elliott, Alexander G. Bobrov, Vladimir L. Motin, Olga Kirillina, Victoria Lao, Richard Calendar, Andrey A. Filippov

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Our analysis of the plague diagnostic phage L-413C genome sequence and structure reveals that L-413C is highly similar and collinear with enterobacteriophage P2, though important differences were found. Of special interest was the mosaic nature of the tail fiber protein H in L-413C, given the differentiating specificity of this phage for Yersinia pestis vs. Yersinia pseudotuberculosis. While the N-terminal 207 and C-terminal 137 amino acids of L-413C display significant homology with the P2 H protein, a large (465 amino acid) middle section appears to be derived from a T4-related H protein, with highest similarity to the T6 and RB32 distal tail fibers. This finding along with appropriate preadsorption experiments suggest that the unique H protein of L-413C may be responsible for the specificity of this phage for Y. pestis, and that the Y. pestis receptors that are recognized and bound by L-413C either do not exist in Y. pseudotuberculosis or have a different structure.

Original languageEnglish (US)
Pages (from-to)85-96
Number of pages12
JournalVirology
Volume372
Issue number1
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

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Keywords

  • Genome sequencing
  • H protein
  • Host-specificity
  • Plague diagnostic bacteriophage
  • Preadsorbtion test
  • Tail fiber
  • Yersinia pestis

ASJC Scopus subject areas

  • Virology

Cite this

Garcia, E., Chain, P., Elliott, J. M., Bobrov, A. G., Motin, V. L., Kirillina, O., Lao, V., Calendar, R., & Filippov, A. A. (2008). Molecular characterization of L-413C, a P2-related plague diagnostic bacteriophage. Virology, 372(1), 85-96. https://doi.org/10.1016/j.virol.2007.10.032