Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the presence of a new LV genotype

E. Susanne Johansson, Bo Niklasson, Robert B. Tesh, Darren R. Shafren, Amelia P.A. Travassos da Rosa, A. Michael Lindberg

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38 Scopus citations

Abstract

Ljungan virus (LV) is a suspected human pathogen recently isolated from bank voles in Sweden. This study describes the genetic characterization of a virus, M1146, which was isolated in 1962 from another vole species (Microtus montanus), trapped in Oregon, USA. Based on antigenic properties, M1146 was postulated previously as a putative member of the family Picornaviridae. The near complete genomic sequence verifies that M1146 is a member of the Picornaviridae, most closely related to LVs isolated in Sweden. The strain M1146 possesses typical LV genomic organization, including a cluster of two 2A homologues. There are significant differences throughout the capsid protein region, while the non-structural region of M1146 is closely related to the Swedish LV genomes. Genetic and phylogenetic analyses show that M1146 represents a new genotype within the distinct LV cluster. Isolation of LV from both Swedish and American voles trapped over a period of 30 years suggests a continuous worldwide presence.

Original languageEnglish (US)
Pages (from-to)837-844
Number of pages8
JournalJournal of General Virology
Volume84
Issue number4
DOIs
StatePublished - Apr 1 2003

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ASJC Scopus subject areas

  • Virology

Cite this

Johansson, E. S., Niklasson, B., Tesh, R. B., Shafren, D. R., Travassos da Rosa, A. P. A., & Lindberg, A. M. (2003). Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the presence of a new LV genotype. Journal of General Virology, 84(4), 837-844. https://doi.org/10.1099/vir.0.18792-0