Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey

Can Bicmen, Nuran Esen, Edward A. Graviss, Natalie Williams-Bouyer, Srinivas V. Ramaswamy, Nuran Yulug

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In recent years, molecular typing methods have been used in epidemiologic studies of Mycobacterium tuberculosis isolates in various areas of the world. However, there have been few data on this issue in Turkey. We describe the molecular characterization of 56 Mycobacterium tuberculosis isolates recovered from individual patients in Izmir and the surrounding area by three different molecular methods. Isolated M. tuberculosis strains were characterized by IS6110 RFLP, spoligotyping and major genetic group designation. In total, 51 RFLP and 35 spoligopatterns were identified. Fourteen (25%) isolates were indicated as low copy number. Based on three genotypic characterization methods together, five clusters with two isolates each were identified. Most of the isolates (98.2%) were assigned as genetic groups 2 or 3. Only one isolate was identified as Beijing family strain (principal genetic group 1). The shared international clades were found to be Beijing-family, var T1 (ST 37), LAM (Latin-American- Mediterranean) 7 (ST 41), LAM 9 (ST 42), Haarlem 1 (ST 47), Haarlem 3 (ST 50) and T1 (ST 53). In this study, IS6110 RFLP, spoligotyping and major genetic group designation were found to be useful methods for molecular epidemiologic studies.

Original languageEnglish (US)
Pages (from-to)229-240
Number of pages12
JournalNew Microbiologica
Volume30
Issue number3
StatePublished - Jul 2007
Externally publishedYes

Fingerprint

Turkey
Mycobacterium tuberculosis
Restriction Fragment Length Polymorphisms
Epidemiologic Studies
Molecular Typing
Beijing

Keywords

  • Molecular characterization
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Bicmen, C., Esen, N., Graviss, E. A., Williams-Bouyer, N., Ramaswamy, S. V., & Yulug, N. (2007). Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey. New Microbiologica, 30(3), 229-240.

Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey. / Bicmen, Can; Esen, Nuran; Graviss, Edward A.; Williams-Bouyer, Natalie; Ramaswamy, Srinivas V.; Yulug, Nuran.

In: New Microbiologica, Vol. 30, No. 3, 07.2007, p. 229-240.

Research output: Contribution to journalArticle

Bicmen, C, Esen, N, Graviss, EA, Williams-Bouyer, N, Ramaswamy, SV & Yulug, N 2007, 'Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey', New Microbiologica, vol. 30, no. 3, pp. 229-240.
Bicmen, Can ; Esen, Nuran ; Graviss, Edward A. ; Williams-Bouyer, Natalie ; Ramaswamy, Srinivas V. ; Yulug, Nuran. / Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey. In: New Microbiologica. 2007 ; Vol. 30, No. 3. pp. 229-240.
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