Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

David R. Higgins, Satya Prakash, Paul Reynolds, Louise Prakash

Research output: Contribution to journalArticle

26 Scopus citations


We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
Issue number2-3
StatePublished - Dec 1983
Externally publishedYes



  • DNA repair
  • Escherichia coli
  • UV sensitivity
  • deletion mutants
  • excision
  • incision
  • pyrimidine dimers
  • yeasts

ASJC Scopus subject areas

  • Genetics

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