Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

David R. Higgins, Satya Prakash, Paul Reynolds, Louise Prakash

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalGene
Volume26
Issue number2-3
DOIs
StatePublished - Dec 1983
Externally publishedYes

Keywords

  • DNA repair
  • Escherichia coli
  • UV sensitivity
  • deletion mutants
  • excision
  • incision
  • pyrimidine dimers
  • yeasts

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this