Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

David R. Higgins, Satya Prakash, Paul Reynolds, Louise Prakash

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalGene
Volume26
Issue number2-3
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Molecular Cloning
Saccharomyces cerevisiae
Gene Deletion
Spores
Genes
Cell Survival
Chromosomes
DNA
Direction compound

Keywords

  • deletion mutants
  • DNA repair
  • Escherichia coli
  • excision
  • incision
  • pyrimidine dimers
  • UV sensitivity
  • yeasts

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. / Higgins, David R.; Prakash, Satya; Reynolds, Paul; Prakash, Louise.

In: Gene, Vol. 26, No. 2-3, 1983, p. 119-126.

Research output: Contribution to journalArticle

Higgins, David R. ; Prakash, Satya ; Reynolds, Paul ; Prakash, Louise. / Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. In: Gene. 1983 ; Vol. 26, No. 2-3. pp. 119-126.
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