Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

David R. Higgins; Satya Prakash; Paul Reynolds; Louise Prakash

doi:10.1016/0378-1119(83)90181-6

Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

David R. Higgins, Satya Prakash, Paul Reynolds, Louise Prakash

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

Original language	English (US)
Pages (from-to)	119-126
Number of pages	8
Journal	Gene
Volume	26
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(83)90181-6
State	Published - Dec 1983
Externally published	Yes

Keywords

DNA repair
Escherichia coli
UV sensitivity
deletion mutants
excision
incision
pyrimidine dimers
yeasts

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(83)90181-6

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title = "Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae",

abstract = "We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.",

keywords = "DNA repair, Escherichia coli, UV sensitivity, deletion mutants, excision, incision, pyrimidine dimers, yeasts",

author = "Higgins, {David R.} and Satya Prakash and Paul Reynolds and Louise Prakash",

note = "Funding Information: We thank Ravi Deshmukhf or help with initial screeningo f transformantas nd RenataP olakowska for the Southerng el shown in Fig. 5. This investigation was supported by grants CA32514 and CA35035t o S.P. and GM19261t o L.P. from NIH. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = dec,

doi = "10.1016/0378-1119(83)90181-6",

language = "English (US)",

volume = "26",

pages = "119--126",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

AU - Higgins, David R.

AU - Prakash, Satya

AU - Reynolds, Paul

AU - Prakash, Louise

N1 - Funding Information: We thank Ravi Deshmukhf or help with initial screeningo f transformantas nd RenataP olakowska for the Southerng el shown in Fig. 5. This investigation was supported by grants CA32514 and CA35035t o S.P. and GM19261t o L.P. from NIH. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/12

Y1 - 1983/12

N2 - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

AB - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

KW - DNA repair

KW - Escherichia coli

KW - UV sensitivity

KW - deletion mutants

KW - excision

KW - incision

KW - pyrimidine dimers

KW - yeasts

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DO - 10.1016/0378-1119(83)90181-6

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AN - SCOPUS:0021048473

SN - 0378-1119

VL - 26

SP - 119

EP - 126

JO - Gene

JF - Gene

IS - 2-3

ER -

Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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