Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein

Hitoshi MIYASAKA, Barun Choudhury, Esther W. HOU, Steven S.‐L LI

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Mouse and human cDNA encoding AES (amino‐terminal enhancer of split) and ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenced. Mouse AES‐1 and AES‐2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino‐terminal region of Drosophila groucho, mouse ESG and human transducin‐like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testis, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.

Original languageEnglish (US)
Pages (from-to)343-352
Number of pages10
JournalEuropean Journal of Biochemistry
Volume216
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Complementary DNA
Muscle
Amino Acids
Brain
Proteins
Placenta
Myocardium
Alternative Splicing
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 15
Chromosomes
Chromosomes, Human, Pair 9
Liver
Hybrid Cells
Cricetulus
Genes
Cells
Drosophila

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. / MIYASAKA, Hitoshi; Choudhury, Barun; HOU, Esther W.; LI, Steven S.‐L.

In: European Journal of Biochemistry, Vol. 216, No. 1, 01.01.1993, p. 343-352.

Research output: Contribution to journalArticle

@article{bd9b401ff46f41f7a6a17f2272145577,
title = "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein",
abstract = "Mouse and human cDNA encoding AES (amino‐terminal enhancer of split) and ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenced. Mouse AES‐1 and AES‐2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50{\%} identity to the amino‐terminal region of Drosophila groucho, mouse ESG and human transducin‐like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testis, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.",
author = "Hitoshi MIYASAKA and Barun Choudhury and HOU, {Esther W.} and LI, {Steven S.‐L}",
year = "1993",
month = "1",
day = "1",
doi = "10.1111/j.1432-1033.1993.tb18151.x",
language = "English (US)",
volume = "216",
pages = "343--352",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein

AU - MIYASAKA, Hitoshi

AU - Choudhury, Barun

AU - HOU, Esther W.

AU - LI, Steven S.‐L

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Mouse and human cDNA encoding AES (amino‐terminal enhancer of split) and ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenced. Mouse AES‐1 and AES‐2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino‐terminal region of Drosophila groucho, mouse ESG and human transducin‐like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testis, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.

AB - Mouse and human cDNA encoding AES (amino‐terminal enhancer of split) and ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenced. Mouse AES‐1 and AES‐2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino‐terminal region of Drosophila groucho, mouse ESG and human transducin‐like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testis, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.

UR - http://www.scopus.com/inward/record.url?scp=0027313018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027313018&partnerID=8YFLogxK

U2 - 10.1111/j.1432-1033.1993.tb18151.x

DO - 10.1111/j.1432-1033.1993.tb18151.x

M3 - Article

C2 - 8365415

AN - SCOPUS:0027313018

VL - 216

SP - 343

EP - 352

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 1

ER -