Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA

Shinji Makino, Ming Fu Chang, Chien Kou Shieh, Toshio Kamahora, David M. Vannier, Sugantha Govindarajan, Michael M C Lai

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Human hepatitis delta (δ) virus (HDV) is a form of defective virus, which infects humans only in the presence of a co-infecting hepatitis B virus (HBV), HDV superinfection in a chronic HBV carrier often results in severe chronic hepatitis and cirrhosis, whereas acute HDV and HBV co-infection is frequently associated with fulminant hepatitis1-3. HDV consists of a 36-nm particle; which contains an envelope with HBV surface antigen, and a nucleocapsid containing the hepatitis δ-antigen (HDAg) and an RNA genome of 1.75 kilobases (kb) (ref. 4). Recently, the genomic RNA from an HDV serially passaged in chimpanzees has been cloned and sequenced in a study which showed that the HDV RNA is a single-stranded circular molecule with properties similar to those of viroid or virusoid5,6. However, it is not known' whether serial passages in chimpanzees had altered the properties of human HDV. Here we report the cloning and sequencing of an HDV RNA isolated directly from a patient with acute δ-hepatitis. The sequence showed considerable divergence (11%) from that of the chimpanzee-adapted HDV. Five open reading frames (ORFs) of more than 100 amino acids in both genomic and anti-genomic sense were found. The largest ORF in antigenomic sense, which can code for 214 amino acids, may correspond to the HDAg.

Original languageEnglish (US)
Pages (from-to)343
Number of pages1
JournalNature
Volume329
Issue number6137
StatePublished - 1987
Externally publishedYes

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Hepatitis Delta Virus
Molecular Cloning
Hepatitis B virus
Pan troglodytes
Hepatitis delta Antigens
RNA
Open Reading Frames
Hepatitis Antigens
Viroids
Serial Passage
Defective Viruses
RNA Sequence Analysis
Amino Acids
Nucleocapsid
Superinfection
Chronic Hepatitis B
Virus Diseases
Chronic Hepatitis
Hepatitis B Surface Antigens
Coinfection

ASJC Scopus subject areas

  • General

Cite this

Makino, S., Chang, M. F., Shieh, C. K., Kamahora, T., Vannier, D. M., Govindarajan, S., & Lai, M. M. C. (1987). Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. Nature, 329(6137), 343.

Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. / Makino, Shinji; Chang, Ming Fu; Shieh, Chien Kou; Kamahora, Toshio; Vannier, David M.; Govindarajan, Sugantha; Lai, Michael M C.

In: Nature, Vol. 329, No. 6137, 1987, p. 343.

Research output: Contribution to journalArticle

Makino, S, Chang, MF, Shieh, CK, Kamahora, T, Vannier, DM, Govindarajan, S & Lai, MMC 1987, 'Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA', Nature, vol. 329, no. 6137, pp. 343.
Makino S, Chang MF, Shieh CK, Kamahora T, Vannier DM, Govindarajan S et al. Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. Nature. 1987;329(6137):343.
Makino, Shinji ; Chang, Ming Fu ; Shieh, Chien Kou ; Kamahora, Toshio ; Vannier, David M. ; Govindarajan, Sugantha ; Lai, Michael M C. / Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. In: Nature. 1987 ; Vol. 329, No. 6137. pp. 343.
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