Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes

Paul L. DeAngelis, John Papaconstantinou, Paul H. Weigel

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Abstract

The hyaluronan (HA) synthase of Group A Streptococci has been identified by transposon mutagenesis and deletion analysis. The genes for the HA synthase and a recently identified UDP-Glc dehydrogenase (Dougherty, B. A., and van de Rijn, I. (1993) J. Biol. Chem. 268, 7118-7124) reside on a contiguous stretch of 3.2-kilobase pair DNA that can direct HA biosynthesis in Enterococcus faecalis and Escherichia coli as well as mutant Streptococcus (DeAngelis, P. L., Papaconstantinou, J., and Weigel, P. H. (1993) J. Biol. Chem. 268, 14568-14571). The synthase contains 395 residues (calculated Mr = 45,063) and migrates on SDS-PAGE with a molecular mass of 42 kDa. E. coli K5, which synthesizes UDP-glucuronic acid for production of its endogenous capsular polysaccharide, can make HA if it contains a plasmid encoding the intact 42-kDa protein. E. coli SURE or χ1448 cells containing the same construct, however, cannot produce HA since these strains cannot make both required sugar nucleotide precursors. The HA synthase is predicted to be an integral membrane protein with four membrane-associated helices, which is consistent with the location of the enzyme activity in Streptococci. There is significant homology between the HA synthase and the Rhizobium nodC gene product, an enzyme that synthesizes chitin-like oligomers. This is the first description at the molecular level of an enzyme shown to synthesize a glycosaminoglycan.

Original languageEnglish (US)
Pages (from-to)19181-19184
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - Sep 15 1993

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Cloning
Streptococcus pyogenes
Molecular Cloning
Hyaluronic Acid
Genes
Streptococcus
Escherichia coli
Enzymes
Uridine Diphosphate Glucuronic Acid
Mutagenesis
Rhizobium
Chitin
Uridine Diphosphate
Enterococcus faecalis
Biosynthesis
Enzyme activity
Molecular mass
Glycosaminoglycans
Oligomers
Sugars

ASJC Scopus subject areas

  • Biochemistry

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Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes. / DeAngelis, Paul L.; Papaconstantinou, John; Weigel, Paul H.

In: Journal of Biological Chemistry, Vol. 268, No. 26, 15.09.1993, p. 19181-19184.

Research output: Contribution to journalArticle

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