Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

Louise Prakash, Dennis Dumais, Renata Polakowska, Giuditta Perozzi, Satya Prakash

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalGene
Volume34
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

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Keywords

  • DNA repair
  • hypersensitivity
  • M13 phage vector
  • pyrimidine dimers
  • radiation sensitivity
  • Recombinant DNA
  • yeast

ASJC Scopus subject areas

  • Genetics

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