Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

Louise Prakash, Dennis Dumais, Renata Polakowska, Giuditta Perozzi, Satya Prakash

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalGene
Volume34
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Molecular Cloning
Saccharomyces cerevisiae
Genes
DNA

Keywords

  • DNA repair
  • hypersensitivity
  • M13 phage vector
  • pyrimidine dimers
  • radiation sensitivity
  • Recombinant DNA
  • yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae. / Prakash, Louise; Dumais, Dennis; Polakowska, Renata; Perozzi, Giuditta; Prakash, Satya.

In: Gene, Vol. 34, No. 1, 1985, p. 55-61.

Research output: Contribution to journalArticle

Prakash, Louise ; Dumais, Dennis ; Polakowska, Renata ; Perozzi, Giuditta ; Prakash, Satya. / Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae. In: Gene. 1985 ; Vol. 34, No. 1. pp. 55-61.
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