Molecular cloning, structure and bait region splice variants of α2-macroglobulin from the soft tick Ornithodoros moubata

Saravanan Thangamani, Christoph Weise, Daniel Sojka, Petr Kopáček

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The sequence of a α2-macroglobulin (α2M) from the soft tick Ornithodoros moubata (TAM) was determined by cloning and sequencing of overlapping polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR products. The TAM cDNA sequence is 4944 bp long and contains one open reading frame coding for a protein precursor composed of 1494 amino-acid residues, including a 24-residue signal sequence. The mature protein is cleaved into two subunits similarly to the C3 and C4 components of complement and fish α2Ms. Phylogeny analysis revealed that TAM is closely related to Limulus α2M and displays the highest similarity to the partial sequence of α2M from hard tick Ixodes scapularis. The comparison of conserved cysteine residues between TAM and human and Limulus α2Ms made it possible to predict the pattern of disulfide bridges and explain the atypical molecular arrangement of TAM. Four variants of the TAM bait region differing only in a short central segment were found; our data indicate that TAM exists as a single-copy gene in the tick genome and its bait region variants likely arise by alternative splicing. TAM is produced by tick hemocytes and it is also significantly expressed in salivary glands. TAM mRNA levels were shown to be up-regulated upon blood meal.

Original languageEnglish (US)
Pages (from-to)841-851
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

Argasidae
Ornithodoros moubata
Ornithodoros
macroglobulins
Macroglobulins
Cloning
Molecular Cloning
Molecular Structure
baits
Limulus
Horseshoe Crabs
molecular cloning
Polymerase chain reaction
Ticks
ticks
Complementary DNA
Genes
polymerase chain reaction
Complement C4
Ixodidae

Keywords

  • α-macroglobulin
  • Bait region
  • Innate immunity
  • Ornithodoros moubata
  • Thioester

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Molecular cloning, structure and bait region splice variants of α2-macroglobulin from the soft tick Ornithodoros moubata. / Thangamani, Saravanan; Weise, Christoph; Sojka, Daniel; Kopáček, Petr.

In: Insect Biochemistry and Molecular Biology, Vol. 33, No. 8, 01.08.2003, p. 841-851.

Research output: Contribution to journalArticle

Thangamani, Saravanan ; Weise, Christoph ; Sojka, Daniel ; Kopáček, Petr. / Molecular cloning, structure and bait region splice variants of α2-macroglobulin from the soft tick Ornithodoros moubata. In: Insect Biochemistry and Molecular Biology. 2003 ; Vol. 33, No. 8. pp. 841-851.
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