TY - JOUR
T1 - Molecular detection of rickettsia felis in different flea species from caldas, colombia
AU - Ramírez-Hernández, Alejandro
AU - Montoya, Viviana
AU - Martínez, Alejandra
AU - Pérez, Jorge E.
AU - Mercado, Marcela
AU - De La Ossa, Alberto
AU - Vélez, Carolina
AU - Estrada, Gloria
AU - Correa, Maria I.
AU - Duque, Laura
AU - Ariza, Juan S.
AU - Henao, Cesar
AU - Valbuena, Gustavo
AU - Hidalgo, Marylin
PY - 2013/9
Y1 - 2013/9
N2 - Rickettsioses caused by Rickettsia felis are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both R. felis and R. typhi. In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify gltA, ompB, and 17kD genes. Positive samples were sequenced to identify the species of Rickettsia. Of 1,341 fleas, Ctenocephalides felis was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in C. felis (minimum infection rates; 5.3%), C. canis (9.2%), and Pulex irritans (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values (> 98%) with R. felis, and all were highly related by phylogenetic analyses. This work shows the first detection of R. felis in fleas collected from animals in Colombia.
AB - Rickettsioses caused by Rickettsia felis are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both R. felis and R. typhi. In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify gltA, ompB, and 17kD genes. Positive samples were sequenced to identify the species of Rickettsia. Of 1,341 fleas, Ctenocephalides felis was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in C. felis (minimum infection rates; 5.3%), C. canis (9.2%), and Pulex irritans (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values (> 98%) with R. felis, and all were highly related by phylogenetic analyses. This work shows the first detection of R. felis in fleas collected from animals in Colombia.
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U2 - 10.4269/ajtmh.12-0698
DO - 10.4269/ajtmh.12-0698
M3 - Article
C2 - 23878183
AN - SCOPUS:84886928399
SN - 0002-9637
VL - 89
SP - 453
EP - 459
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -