Molecular diagnosis of bovine tuberculosis in bovine and human samples: Implications for zoonosis

Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo, Hossam M. Ashour

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

Original languageEnglish (US)
Pages (from-to)527-535
Number of pages9
JournalFuture Microbiology
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

Keywords

  • bovine tuberculosis
  • molecular diagnosis
  • zoonotic transmission

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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