Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas

Marcio R T Nunes, Amelia P A Travassos Da Rosa, Scott Weaver, Robert B. Tesh, Pedro F C Vasconcelos

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To date, no molecular studies on group C viruses (Bunyaviridae, Orthobunyavirus) have been published. We determined the complete small RNA (SRNA) segment and partial medium RNA segment nucleotide sequences for 13 group C members. The full-length SRNA sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. Based on the 705 nucleotides of the N gene, group C members were distributed into three major phylogenetic groups, with the exception of Madrid virus, which was placed outside of these three groups. Analysis of the Caraparu virus strain BeH 5546 revealed that it has an SRNA sequence nearly identical to that of Oriboca virus and is a natural reassortant virus. In addition, analysis of 345 nucleotides of the Gn gene for eight group C viruses and for strain BeH 5546 revealed a different phylogenetic topology, suggesting a reassortment pattern among them. These findings represent the first evidence for natural reassortment among the group C viruses, which include several human pathogens. Furthermore, our genetic data corroborate previous relationships determined using serologic assays (complement fixation, hemagglutination inhibition, and neutralization tests) and suggest that a combination of informative molecular, serological, and ecological data is a helpful tool to understand the molecular epidemiology of arboviruses.

Original languageEnglish (US)
Pages (from-to)10561-10570
Number of pages10
JournalJournal of Virology
Volume79
Issue number16
DOIs
StatePublished - Aug 2005

Fingerprint

Orthobunyavirus
Bunyaviridae
molecular epidemiology
Molecular Epidemiology
Viruses
viruses
Nucleotides
nucleotides
nucleotide sequences
Reassortant Viruses
RNA
Hemagglutination Inhibition Tests
Arboviruses
Neutralization Tests
arboviruses
hemagglutination inhibition test
phylogeny
neutralization tests
Genes
topology

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas. / Nunes, Marcio R T; Travassos Da Rosa, Amelia P A; Weaver, Scott; Tesh, Robert B.; Vasconcelos, Pedro F C.

In: Journal of Virology, Vol. 79, No. 16, 08.2005, p. 10561-10570.

Research output: Contribution to journalArticle

Nunes, Marcio R T ; Travassos Da Rosa, Amelia P A ; Weaver, Scott ; Tesh, Robert B. ; Vasconcelos, Pedro F C. / Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas. In: Journal of Virology. 2005 ; Vol. 79, No. 16. pp. 10561-10570.
@article{29b7899225544e399809d2ad2e7437c8,
title = "Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas",
abstract = "To date, no molecular studies on group C viruses (Bunyaviridae, Orthobunyavirus) have been published. We determined the complete small RNA (SRNA) segment and partial medium RNA segment nucleotide sequences for 13 group C members. The full-length SRNA sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. Based on the 705 nucleotides of the N gene, group C members were distributed into three major phylogenetic groups, with the exception of Madrid virus, which was placed outside of these three groups. Analysis of the Caraparu virus strain BeH 5546 revealed that it has an SRNA sequence nearly identical to that of Oriboca virus and is a natural reassortant virus. In addition, analysis of 345 nucleotides of the Gn gene for eight group C viruses and for strain BeH 5546 revealed a different phylogenetic topology, suggesting a reassortment pattern among them. These findings represent the first evidence for natural reassortment among the group C viruses, which include several human pathogens. Furthermore, our genetic data corroborate previous relationships determined using serologic assays (complement fixation, hemagglutination inhibition, and neutralization tests) and suggest that a combination of informative molecular, serological, and ecological data is a helpful tool to understand the molecular epidemiology of arboviruses.",
author = "Nunes, {Marcio R T} and {Travassos Da Rosa}, {Amelia P A} and Scott Weaver and Tesh, {Robert B.} and Vasconcelos, {Pedro F C}",
year = "2005",
month = "8",
doi = "10.1128/JVI.79.16.10561-10570.2005",
language = "English (US)",
volume = "79",
pages = "10561--10570",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "16",

}

TY - JOUR

T1 - Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas

AU - Nunes, Marcio R T

AU - Travassos Da Rosa, Amelia P A

AU - Weaver, Scott

AU - Tesh, Robert B.

AU - Vasconcelos, Pedro F C

PY - 2005/8

Y1 - 2005/8

N2 - To date, no molecular studies on group C viruses (Bunyaviridae, Orthobunyavirus) have been published. We determined the complete small RNA (SRNA) segment and partial medium RNA segment nucleotide sequences for 13 group C members. The full-length SRNA sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. Based on the 705 nucleotides of the N gene, group C members were distributed into three major phylogenetic groups, with the exception of Madrid virus, which was placed outside of these three groups. Analysis of the Caraparu virus strain BeH 5546 revealed that it has an SRNA sequence nearly identical to that of Oriboca virus and is a natural reassortant virus. In addition, analysis of 345 nucleotides of the Gn gene for eight group C viruses and for strain BeH 5546 revealed a different phylogenetic topology, suggesting a reassortment pattern among them. These findings represent the first evidence for natural reassortment among the group C viruses, which include several human pathogens. Furthermore, our genetic data corroborate previous relationships determined using serologic assays (complement fixation, hemagglutination inhibition, and neutralization tests) and suggest that a combination of informative molecular, serological, and ecological data is a helpful tool to understand the molecular epidemiology of arboviruses.

AB - To date, no molecular studies on group C viruses (Bunyaviridae, Orthobunyavirus) have been published. We determined the complete small RNA (SRNA) segment and partial medium RNA segment nucleotide sequences for 13 group C members. The full-length SRNA sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. Based on the 705 nucleotides of the N gene, group C members were distributed into three major phylogenetic groups, with the exception of Madrid virus, which was placed outside of these three groups. Analysis of the Caraparu virus strain BeH 5546 revealed that it has an SRNA sequence nearly identical to that of Oriboca virus and is a natural reassortant virus. In addition, analysis of 345 nucleotides of the Gn gene for eight group C viruses and for strain BeH 5546 revealed a different phylogenetic topology, suggesting a reassortment pattern among them. These findings represent the first evidence for natural reassortment among the group C viruses, which include several human pathogens. Furthermore, our genetic data corroborate previous relationships determined using serologic assays (complement fixation, hemagglutination inhibition, and neutralization tests) and suggest that a combination of informative molecular, serological, and ecological data is a helpful tool to understand the molecular epidemiology of arboviruses.

UR - http://www.scopus.com/inward/record.url?scp=23244459533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23244459533&partnerID=8YFLogxK

U2 - 10.1128/JVI.79.16.10561-10570.2005

DO - 10.1128/JVI.79.16.10561-10570.2005

M3 - Article

C2 - 16051848

AN - SCOPUS:23244459533

VL - 79

SP - 10561

EP - 10570

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 16

ER -